Vessel Mounting Technique
Vessel Mounting Technique - Related Videos
03:43
A Whole-Mount Organ Staining Technique for Three-Dimensional Imaging of the Kidney
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2025
This video demonstrates an assay for the whole-mount staining of murine kidneys using a tissue-clearing method called CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) and immunofluorescent staining. The kidney is subjected to tissue clearing, wherein the kidney is delipidated, decolorized, and treated with a refractive index matching solution to make the tissue transparent for optimum three-dimensional imaging. The tissue is stained with fluorescently labeled...
04:31
Assessing Vessel Perfusion via Angiography: A Technique to Introduce Contrast Agent in Descending Aorta to Visualize Blood Vessel Perfusion in Rabbit Model
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2025
This video describes the technique for performing angiography by injecting a contrast agent into the descending aorta to visualize blood vessel perfusion in a rabbit model. This technique helps to understand the perfusion in blood vessels with blockages and after treatment during recovery.
12:41
A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
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Cited by 1 •
2017
This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.
07:32
Purification of Mouse Brain Vessels
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Cited by 90 •
2015
We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.
13:10
Stability of Floating Vessels
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2023
Source: Alexander S Rattner and Kevin Rao Li Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA The objective of this experiment is to demonstrate the phenomenon of stability of floating vessels - the ability to self-right when rolled over to the side by some external force. Careful design of hull shapes and internal mass distribution enables seagoing vessels to be stable with low drafts (submerged depth of hull), improving vessel...
FAQs
These regulated conditions provide distinct mechanical and environmental inputs to the isolated vessel. Researchers can examine how vascular tissue responds when pressure or flow changes, or when temperature and diameter are controlled. Comparing responses under these conditions helps relate vessel behavior to mechanical forces and supports analysis of changes in vascular tone and blood pressure regulation.
Supports or cannulas keep the dissected vessel positioned for observation and measurement, while the physiological solution maintains the controlled preparation. Together, these components allow researchers to regulate the experimental environment without losing access to the vessel. This arrangement makes it possible to monitor structural and functional responses using microscopy or force recording.
The preparation can be used to examine responses to drugs, hormones, neural signals, and mechanical forces. These inputs represent different influences on vascular behavior, allowing researchers to compare how the vessel reacts to chemical, neural, or physical stimulation. The resulting measurements can reveal changes in vessel diameter, vascular tone, or force-related behavior.
Preparation begins with careful dissection of the vessel, followed by attachment to supports or cannulas. The mounted tissue is then maintained in a physiological solution while conditions such as pressure, flow, temperature, or diameter are regulated. Researchers can subsequently apply selected signals and record structural or functional responses through microscopy or force measurements.
It is useful when researchers need direct measurements from vascular tissue rather than indirect observations. The method supports investigations of vascular tone, endothelial function, blood pressure regulation, and disease-related changes in smooth muscle or vessel walls. Because experimental conditions can be controlled, investigators can examine how isolated vessels respond to defined biological and mechanical influences.
A mounted preparation can provide observations of vessel structure and measurements of functional responses. Microscopy may document changes in the vessel, while force recording can capture mechanical behavior. Depending on the signals and conditions applied, the results can help characterize vascular tone, endothelial function, responses to mechanical forces, and alterations associated with disease.