Wide-field Microscopy
Wide-field Microscopy - Related Videos
06:42
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Cited by 32 •
2013
We review our recent results on the integration of fluorescent microscopy and imaging flow cytometry tools on a cell-phone using compact and cost-effective opto-fluidic attachments. These cell-phone based micro-analysis devices might be useful for cytometric analysis, such as performing various cell counting tasks as well as for high-throughput screening of e.g., water samples in resource limited settings.
06:48
A Multimodal Wide-Field Fourier-Transform Raman Microscope
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2025
A wide-field Fourier-transform microscope, based on a compact and ultra-stable birefringent interferometer, allows the parallel acquisition of spectra for all pixels of a 2D detector. The time-domain approach enables the disentanglement of photoluminescence and Raman signals, and allows rapid Raman mapping (~5 ms/pixel) with ~1-µm spatial and 23-cm-1 spectral resolution.
10:05
A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity
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Cited by 18 •
2017
This protocol presents a method for creating a large unilateral craniotomy over the temporal and parietal regions of the mouse cerebral cortex. This is especially useful for real time imaging over an expansive area of a cortical hemisphere.
06:45
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
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Cited by 9 •
2022
The present protocol describes making a large (6 x 3 mm2) cranial window using food wrap, transparent silicone, and cover glass. This cranial window allows in vivo wide-field and two-photon calcium imaging experiments in the same mouse.
FAQs
Excitation light reaches the specimen and interacts with fluorescently labeled molecules. Those molecules emit light at the site of labeling, and the objective lens collects the emitted signal for camera detection. Because the camera records the field simultaneously, the resulting image can reveal where labeled proteins or other molecules are positioned across cells, tissues, or broader biological regions.
Simultaneous detection allows the instrument to capture information from the entire illuminated field at once rather than building an image from separately recorded locations. This supports rapid observation of broad cellular or tissue regions and helps researchers examine spatial organization while retaining a practical balance between image coverage and acquisition speed.
Fluorescence labeling connects emitted light with the locations of selected molecules in a specimen. In wide-field microscopy, this makes protein localization visible across the recorded field and can relate molecular distribution to cell shape, tissue organization, or other structures. The approach therefore links molecular position with larger-scale biological context in a single image.
Illuminating the entire specimen field makes the technique suitable for biological samples that extend across broad areas, including cells, tissues, and microbial populations. Researchers can observe overall organization rather than focusing only on a small selected region. This broad view is useful for assessing patterns such as tissue arrangement, cell morphology, and microbial growth across the field.
A typical acquisition begins by positioning the biological specimen within the imaging field and selecting the relevant optical view. For fluorescence imaging, excitation light is directed through or reflected from the sample. Emitted light is then collected by the objective lens and recorded by a camera, producing an image that can be examined visually or analyzed quantitatively.
Researchers may choose this approach when they need rapid visualization over a relatively broad area without making image coverage the sole priority. It is useful for routine examination of cell shape, protein localization, microbial growth, and tissue organization. The combination of broad coverage, acquisition speed, and experimental simplicity also supports repeated biological imaging and quantitative analysis.
Images from this technique can serve as data for measuring or comparing biological patterns across the recorded field. Depending on the specimen and labeling, analysis may address cell shape, the distribution of proteins, microbial growth, or tissue organization. Recording many structures simultaneously helps preserve spatial relationships that are relevant when interpreting biological organization and change.