Zebrafish Microinjection
Zebrafish Microinjection - Related Videos
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Zebrafish Microinjection Techniques
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2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
03:10
Microinjection of Bacteria into the Caudal Vein of Zebrafish Embryos
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2026
Source: Shi, J., et al., Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates the procedure for preparing an Agrobacterium tumefaciens culture by inducing virulence gene expression with acetosyringone, followed by antibiotic removal through washing and resuspension, to generate a transformation-ready suspension for gene delivery into plant tissues.
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
03:58
Embryo Microinjection: A Technique to Deliver a Compound into the Zebrafish Yolk
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2023
This video describes a technique of Embryo microinjection to deliver compounds into Zebrafish yolk sac.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
FAQs
The cargo selected for zebrafish microinjection determines the biological question it can address. mRNA, DNA, morpholinos, and CRISPR reagents support gene-expression manipulation or the creation of mutant and transgenic models, whereas fluorescent tracers support cell-lineage tracing. This distinction helps match the injected material to an intended outcome rather than treating all injections as interchangeable.
Injecting at the one-cell stage places the material into the embryo before substantial development has occurred. This timing makes the intervention relevant to later developmental processes and gene-function studies. It also provides an early point at which researchers can introduce reagents intended to influence development, generate models, or mark cells for subsequent lineage-tracing studies.
Yolk, cytoplasm, and a developing cell are distinct deposition sites, so the selected destination must match the experimental objective. Material placed into these regions can support different types of developmental, gene-function, or lineage studies. Specifying the target site therefore adds an important experimental variable beyond simply choosing which material to inject.
A typical workflow positions a zebrafish embryo under a microscope, uses a fine glass micropipette to penetrate the chorion, and deposits a precise amount of the selected material into the yolk, cytoplasm, or developing cell. The procedure combines visual targeting with controlled delivery, allowing the injected cargo and its destination to align with the study's biological aim.
The essential setup includes zebrafish embryos, a microscope for visual guidance, and a fine glass micropipette for penetrating the chorion and delivering material. Researchers also prepare the cargo appropriate to the experiment, such as mRNA, DNA, morpholinos, CRISPR reagents, or fluorescent tracers. These components support precise delivery at an early developmental stage.
In biology, the technique supports studies of embryonic development and gene function by enabling gene-expression manipulation, transgenic or mutant model generation, and cell-lineage tracing. These capabilities also extend its use to investigations of disease mechanisms and potential therapeutic strategies. The resulting embryos or models provide experimental systems for connecting introduced materials with developmental or biological outcomes.