Human Fibroblasts
Human Fibroblasts - Related Videos
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
03:32
Generating Neurons by Reprogramming Human Dermal Fibroblast Cells
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2025
The video demonstrates the generation of neurons from adult human dermal fibroblasts (aHDFs). The aHDFs are reprogrammed using a lentiviral vector carrying genes for neuronal transcription factors and short hairpin RNAs (shRNAs). The shRNAs inhibit the expression of a neuronal transcriptional repressor, and the neuronal transcription factors activate genes to differentiate aHDFs into neurons. These reprogrammed aHDFs are cultured in an early and a late neuronal conversion medium to generate...
03:33
Generating a 3D Co-Culture Model of Human Corneal Fibroblasts and Neurons
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2025
This video demonstrates a technique to generate a three-dimensional co-culture model system of primary human corneal fibroblasts and differentiated neuronal cells. This co-culture system can be used to study the stromal-nerve interactions.
06:02
Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens
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Cited by 17 •
2016
Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.
10:04
Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
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Cited by 2 •
2017
Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.
FAQs
Human fibroblasts can influence tumors through both matrix remodeling and secreted signals. In a tumor-associated state, their activity may alter the extracellular environment and communication among local cells, affecting cancer-cell growth, invasion, immune responses, and sensitivity to treatment. This makes fibroblast behavior a mechanistic link between stromal changes and tumor phenotypes studied in cancer models.
Collagen and fibronectin production gives fibroblasts a way to alter the physical and biochemical context around tumor cells. Because fibroblasts respond to mechanical as well as biochemical signals, changes in their environment can modify proliferation, migration, and secretory activity. Researchers therefore examine matrix remodeling as a potential driver of altered tumor-cell behavior.
Compared with fibroblasts involved in ordinary tissue maintenance and repair, cancer-associated fibroblasts are investigated for tumor-supportive activities. These include reshaping the extracellular matrix and releasing signaling factors that influence tumor growth, invasion, immune responses, and treatment sensitivity. The comparison helps researchers separate general stromal functions from changes associated with the tumor microenvironment.
Cultured fibroblasts provide a controllable system for examining their proliferation, migration, matrix production, and secretory activity. Co-culture models extend this approach by placing fibroblasts in interaction with cancer-related cells, allowing researchers to investigate tumor-stroma communication. Comparing these model systems can clarify how fibroblast activity contributes to changes in local tumor behavior.
A fibroblast-based model is useful when the research question concerns interactions between connective-tissue cells and tumor cells rather than cancer cells alone. Investigators can use cultured fibroblasts or co-culture systems to examine extracellular-matrix remodeling, signaling-factor release, and cellular responses. These models are especially relevant for studying growth, invasion, immune effects, and treatment sensitivity.
Studies can identify how fibroblast activity is associated with tumor growth, invasion, immune responses, or reduced treatment sensitivity. They can also show how extracellular-matrix changes and released signaling factors modify the local cellular environment. Such findings help researchers clarify tumor-stroma interactions and may point to fibroblast-related processes as potential therapeutic targets.