Tissue Processing Protocol
Tissue Processing Protocol - Related Videos
08:31
Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
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Cited by 14 •
2017
This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.
15:52
Postproduction Processing of Electrospun Fibres for Tissue Engineering
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Cited by 30 •
2012
Electrospun scaffolds can be processed post production for tissue engineering applications. Here we describe methods for spinning complex scaffolds (by consecutive spinning), for making thicker scaffolds (by multi-layering using heat or vapour annealing), for achieving sterility (aseptic production or sterilisation post production) and for achieving appropriate biomechanical properties.
13:08
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture
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Cited by 80 •
2013
A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.
02:41
Cryopreserved Tissue Section Fixation and Staining: A Procedure to Process Frozen Brain Tissue Sections for Laser Microdissection
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2023
This video presents the fixation and staining method to process frozen mouse brain tumor tissue. The processed tissue helps differentiate and obtain a specific population of cells during subsequent laser capture microdissection.
05:30
The Combination of Mechanically Isolated Stromal Vascular Fraction and Fibrin Hydrogel: A Processing Protocol
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Cited by 1 •
2023
Mechanically isolated stromal vascular fraction (SVF) in combination with a fibrin hydrogel offers an easy and efficient carrier for viable adipose-derived stromal cells for various indications, including tissue engineering and or wound healing purposes. Here, we present the preparation of a mechanical SVF (mSVF)-fibrin hydrogel construct for translational research and clinical application.
FAQs
Fixation conditions can affect both tissue morphology and the detectability of antigens used in immunohistochemistry. Excessive or insufficient fixation may therefore change how cellular structures appear or how strongly biomarkers are detected. In cancer research, controlling fixation time and related handling conditions helps preserve interpretable tumor architecture and supports more reliable comparisons among specimens.
Dehydration removes water from the specimen, while clearing replaces that water-removal environment with a medium compatible with the embedding material. If these stages are not properly matched, the tissue may not be prepared consistently for support and sectioning. Their controlled sequence is therefore important for maintaining specimen integrity during later microscopic analysis.
Tumor and surrounding tissue should be processed under comparable conditions because handling differences can influence morphology and biomarker detection independently of true biological differences. Standardized fixation, reagent conditions, and processing times reduce such technical variation. This improves the interpretability of histopathology and immunohistochemistry comparisons within cancer research experiments.
The workflow proceeds through stabilization by fixation, water removal by dehydration, replacement with a compatible medium during clearing, and support through paraffin or another embedding material. The supported specimen can then be sectioned and stained for microscopic evaluation. Maintaining this order prepares tissue architecture and biomarker distribution for downstream analysis.
Fixation time, reagent conditions, and specimen handling require close control because each can influence morphology, antigen detection, and downstream reliability. The embedding support and preparation for sectioning also affect whether tissue can be examined consistently. Monitoring these variables is especially important when multiple cancer specimens must be compared across an experiment or study.
Processed specimens support histopathology, which examines tissue structure, and immunohistochemistry, which assesses biomarker distribution. The resulting sections can be used to compare tumors with adjacent or surrounding tissue while preserving relevant cellular architecture. Processing also prepares specimens for molecular analysis, making the workflow useful across complementary cancer research investigations.