Tumor Stroma
Tumor Stroma - Related Videos
07:20
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
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Cited by 22 •
2020
A novel three-dimensional spheroid model based on the heterotypic interaction of tumor cells and stromal fibroblasts is established. Here, we present coculture of tumor cells and stromal fibroblasts, time-lapse imaging, and confocal microscopy to visualize the formation of spheroids. This three-dimensional model offers a pertinent platform to study tumor-stroma interactions and test cancer therapeutics.
01:24
Protein Transport to the Stroma
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2023
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function. Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
08:40
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
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Cited by 24 •
2020
This protocol presents a 3D biomimetic model with accompanying fibrotic stromal compartment. Prepared with physiologically relevant hydrogels in ratios mimicking the bio-physical properties of the stromal extracellular matrix, an active mediator of cellular interactions, tumor growth and metastasis.
08:04
Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
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Cited by 51 •
2011
We present a novel approach to quantify nanoparticle localization in the vasculature of human xenografted tumors using dynamic, real-time intravital imaging in an avian embryo model.
07:59
Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
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Cited by 3 •
2021
Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.
FAQs
Communication occurs through cytokines, growth factors, and physical remodeling of the surrounding tissue. These signals allow stromal components to influence cancer-cell behavior rather than acting as passive support. In turn, the resulting interactions can promote tumor growth, invasion, angiogenesis, immune suppression, and reduced sensitivity to treatment, making cellular signaling a central mechanism in tumor progression.
Each stromal component can affect tumor behavior through a different function. Extracellular matrix provides a remodeled physical environment, cancer-associated fibroblasts contribute to tissue support and signaling, blood vessels support angiogenesis, and immune cells participate in local immune regulation. Their combined activity creates conditions that may support invasion, immune suppression, growth, and treatment resistance.
Physical remodeling changes the environment through which cancer cells move and receive signals. At the same time, stromal communication can stimulate angiogenesis, alter immune activity, and reinforce conditions that protect malignant cells from treatment effects. Consequently, tumor behavior and therapeutic response may depend not only on cancer-cell properties but also on the surrounding tissue interactions.
A cancer-cell-focused strategy primarily addresses the malignant population, whereas a stromal strategy examines the supporting environment that can sustain tumor progression. Targeting the stroma seeks to modify extracellular matrix, fibroblast activity, blood-vessel formation, immune suppression, or related signaling. This broader approach can reveal therapeutic opportunities that would be missed by studying malignant cells in isolation.
Researchers examine stromal features and their relationships with cancer-cell behavior to determine how the local environment affects tumor growth and spread. Analysis can focus on extracellular matrix, cancer-associated fibroblasts, blood vessels, immune cells, and their signaling interactions. The resulting information supports evaluation of prognostic biomarkers, therapeutic targets, and features associated with treatment response.
Stromal features can help indicate how a tumor may behave and how it may respond to therapy. Patterns involving the extracellular matrix, fibroblasts, vessels, immune cells, or local signaling may serve as prognostic biomarkers or response-related indicators. Incorporating these features therefore adds environmental context to assessments based solely on malignant-cell characteristics.
Such strategies are relevant when stromal interactions contribute to tumor growth, invasion, immune suppression, angiogenesis, or resistance to treatment. By altering the surrounding environment rather than focusing exclusively on cancer cells, researchers can investigate whether treatment response improves or whether progression-related signals are reduced. This approach also helps connect mechanistic findings with therapeutic development in cancer research.