Zebrafish Engraftment
Zebrafish Engraftment - Related Videos
04:36
Adenocarcinoma Engraftment: A Method to Implant Cancer Cells into the Murine Lungs
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2023
This video describes a method to engraft lung adenocarcinoma cells in murine lungs by preconditioning the airways with fibrosis-inducing agent–bleomycin. This method significantly enhances orthotopic engraftment of lung cancer cells into murine...
10:52
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
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Cited by 1 •
2018
Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
08:15
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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Cited by 4 •
2009
We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.
09:39
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
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Cited by 16 •
2014
Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42 •
2011
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
FAQs
Placement at a defined anatomical site gives the transplanted cells a consistent location in which to be observed. Researchers can then assess whether the cells survive, proliferate, interact with host tissues, or disseminate from that site. This spatial design helps connect tumor-cell behavior with outcomes occurring across a living organism.
Fluorescent labeling makes transplanted tumor cells distinguishable during fluorescence microscopy. Researchers can track where the cells are located and assess changes associated with survival, proliferation, invasion, or dissemination. This readout turns otherwise difficult-to-observe behavior inside the host into visible measurements that support analysis of cancer samples and experimental conditions.
Zebrafish engraftment can connect several cancer phenotypes within one living model. The system can be used to examine invasion, metastatic dissemination, angiogenesis, and cellular proliferation, while also revealing interactions between transplanted cells and host tissues. Considering these outcomes together provides broader context than evaluating tumor-cell behavior only in isolation.
Researchers introduce tumor cells or patient-derived cancer tissue into zebrafish embryos or larvae at a defined anatomical site. The transplanted material is labeled so it can be visualized, and fluorescence microscopy is then used to track the cells. Observations can address survival, proliferation, interactions with host tissues, and dissemination.
These stages support rapid analysis while requiring relatively small numbers of transplanted cells. The approach also has a relatively low experimental cost, making it practical for examining tumor behavior and responses to anticancer compounds. Speed, limited cell requirements, and cost can facilitate experiments connecting cellular mechanisms with whole-organism outcomes.
The method evaluates how cancer cells behave in a living organism and how they respond to anticancer compounds. It can contribute to preclinical drug evaluation by linking treatment responses with observed tumor-cell behavior. When patient-derived cancer tissue is used, the same framework also supports personalized cancer research.