Dispersive Ir Spectrometer

A dispersive IR spectrometer is an analytical instrument that measures how a sample absorbs infrared radiation across different wavelengths, revealing molecular structure and chemical composition. It uses a broadband infrared source and a monochromator, such as a prism or diffraction grating, to separate the radiation into individual wavelengths; the instrument then scans the spectrum and records transmitted intensity with a detector. The resulting absorption bands correspond to molecular vibrations, including stretching and bending of chemical bonds. In chemistry, dispersive IR spectrometers support functional-group identification, compound characterization, purity assessment, and reaction monitoring, although modern Fourier-transform instruments often provide faster measurements.

Dispersive Ir Spectrometer - Related Videos

Education

JoVE Core - Analytical Chemistry

IR Spectrometers

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2024

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

Research

JoVE Journal - Bioengineering

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

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Cited by 40 •

2015

Here we present a protocol to build a rapid Brillouin spectrometer. Cascading virtually imaged phase array (VIPA) etalons achieve a measurement speed more than 1,000 times faster than traditional scanning Fabry-Perot spectrometers. This improvement provides the means for Brillouin analysis of tissue and biomaterials at low power levels in vivo.

Mass Spectrometers

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2024

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions: Conversion of the gas-phase analyte atoms/molecules into a beam of positive or negative charged ions by ionization. Separation of the charged species based on their mass-to-charge ratio. Recording the relative abundance of each type of ion. In the ionization chamber of...

Research

JoVE Journal - Immunology and Infection

Assessing Biofilm Dispersal in Murine Wounds

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Cited by 2 •

2021

Here, we describe ex vivo and in vivo methods for assessing bacterial dispersal from a wound infection in mice. This protocol can be utilized to test the efficacy of topical antimicrobial and anti-biofilm therapies, or to assess the dispersal capacity of different bacterial strains or species.

Distribution and Dispersion

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2026

Ecology is the study of how organisms interact with their environment and with one another. An important aspect of ecology is understanding where species are found and how individuals are distributed within those areas. The geographic range of a species refers to the total area where its members are located, while dispersion describes the pattern of spacing of individuals within that range.Geographic Range and Dispersion PatternsWithin a species’ geographic range, individuals may be distributed...

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