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High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
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Bioengineering
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JoVE Journal Bioengineering
High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

DOI:
10.3791/53468-v

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13:31 min

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December 22, 2015

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, ,
1Fischell Department of Bioengineering,University of Maryland, 2Wellman Center for Photomedicine,Harvard Medical School, Massachusetts General Hospital, 3The Harvard-MIT Division of Health Sciences and Technology,Massachusetts Institute of Technology

Chapters

  • 00:05Title
  • 00:49Setup of Fiber and the Electron Multiplied Charge Coupled Device (EMCCD) Camera
  • 01:49The Horizontal Stage of the Spectrometer
  • 06:19The Vertical Stage of the Spectrometer
  • 08:36Combination of the Two Stages
  • 10:12Measuring the Brillouin Shift
  • 11:46Results: Brillouin Spectra of Methanol, Ethanol, and Polystyrene
  • 12:48Conclusion

Summary

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Here we present a protocol to build a rapid Brillouin spectrometer. Cascading virtually imaged phase array (VIPA) etalons achieve a measurement speed more than 1,000 times faster than traditional scanning Fabry-Perot spectrometers. This improvement provides the means for Brillouin analysis of tissue and biomaterials at low power levels in vivo.

Tags

Keywords: Brillouin SpectroscopyHigh-speedHigh-resolutionSub-GHzVIPAMaterial CharacterizationStructural MonitoringEnvironmental SensingBiomedical ApplicationsViscoelastic Properties

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