Dual-inlet System
Dual-inlet System - Related Videos
07:33
In vivo Dual Substrate Bioluminescent Imaging
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Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
08:35
Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
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Cited by 12 •
2015
Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.
05:48
Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
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Cited by 1 •
2014
This video presents a protocol for the mass spectrometrical analysis of volatile and oxidation sensitive compounds using electron impact ionization. The presented technique is especially of interest for inorganic chemists, working with metal organyls, silanes, or phosphanes which have to be handled using inert conditions, such as the Schlenk technique.
10:30
Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion
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Cited by 4 •
2013
Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.
03:16
Dual Fluorescence Imaging to Identify Differentiated Oligodendrocytes
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2025
Source: Schott, J. T., et al. Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning. J. Vis. Exp. (2016). This video demonstrates the use of dual fluorescence imaging to evaluate the differentiation of oligodendrocytes from precursor cells (OPCs) isolated from a rat pup brain. The process includes fixing the cells, labeling specific markers with primary and fluorophore-conjugated secondary antibodies, and...
FAQs
Alternating gas streams lets the detector compare the sample and reference under closely similar instrument conditions. Because measurements occur through repeated switching rather than through isolated runs, changes in instrument behavior have less influence on the comparison. This improves measurement stability and helps reveal relatively subtle differences in gas composition or isotope ratios.
Separate inlet paths allow the sample gas and reference gas to be managed independently before they reach the instrument. Controlled valves can alternate the streams or admit them sequentially, while pressure is regulated for each path. This control preserves a consistent comparison framework and supports precise evaluation of differences between the two materials.
In isotope-ratio mass spectrometry, the system repeatedly switches between a sample gas and an isotopically characterized reference. The detector can therefore compare their isotope compositions while instrument conditions remain as consistent as possible. Repeated comparison improves precision and stability, which is important when the measured isotopic variation is small.
A measurement begins by directing the sample and reference gases into their separate inlet paths. Valves then alternate the streams or admit them sequentially under controlled pressure, allowing the instrument to receive each gas in a defined sequence. The detector records the resulting differences in composition, creating a direct comparison between the materials.
Researchers choose this approach when they need a precise comparison with a reference material, especially for isotope-ratio measurements. The method is relevant when small compositional differences must be distinguished from changes in instrument conditions. It can support studies of reaction pathways, material sources, environmental processes, and subtle isotopic variations.
Results from dual-inlet measurements can connect isotopic or compositional differences with the origin and transformation of materials. In chemistry, this supports investigation of reaction pathways and material sources, while environmental studies can examine processes associated with isotopic variation. The comparison with a characterized reference strengthens interpretation of these differences.