Purity Determination
Purity Determination - Related Videos
08:02
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
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Cited by 1 •
2020
Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.
12:17
Detection and Isolation of Apoptotic Bodies to High Purity
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Cited by 29 •
2018
A workflow using flow cytometry or differential centrifugation is developed to detect, quantify and isolate apoptotic bodies from an apoptotic sample to high purity.
12:06
Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants
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Cited by 6 •
2017
A protocol for the synthesis of high purity nonsymmetric dialkylphosphinic acid extractants is presented, taking (2,3-dimethylbutyl)(2,4,4'-trimethylpentyl)phosphinic acid as an example.
07:54
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
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Cited by 22 •
2017
Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.
08:49
Photometric Protein Determination
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2023
Measuring the concentration is a fundamental step of many biochemical assays. Photometric protein determination takes advantage of the fact that the more a sample contains light-absorbing substances, the less the light will transmit through it. Since the relationship between concentration and absorption is linear, this phenomenon can be used to measure the concentration in samples where it is unknown. This video describes the basics of photometric protein determination and introduces the...
FAQs
Melting-point analysis can reveal contamination through a changed physical response. A chemist measures the sample’s melting behavior and compares it with the value expected for the pure compound. A discrepancy indicates that the sample may contain additional material, making this approach useful as a property-based check during chemical identification and quality control.
Chromatography can indicate additional components in a sample alongside the desired compound. This makes it useful when purity determination requires evidence that more than one substance is present, rather than relying only on a single bulk property. The resulting information supports evaluation of sample composition in analytical chemistry and quality-control work.
Chemical measurements such as titration and gravimetric analysis address a different aspect of purity determination from a physical-property comparison. They can measure the amount of analyte, while spectroscopy can reveal changes associated with contamination. These approaches help connect sample composition with quantitative results, which is important when identification alone is not sufficient.
A practical workflow begins by selecting a measurable property or chemical response relevant to the compound, then comparing the sample result with the expected result for the pure substance. Depending on the question, the analysis may focus on detecting additional components, measuring analyte amount, or identifying contamination-related changes. The selected outcome determines how purity is judged.
Purity determination supports pharmaceutical manufacturing by assessing whether materials have the expected composition. In industrial quality assurance, the same principle helps maintain consistent composition and performance. Because the process also supports analytical research, it serves both routine control and investigations that require dependable quantitative results from chemically characterized materials.
Within chemistry, purity determination connects identification, quality control, and quantitative analysis. A sample can appear to be the expected substance yet still produce unreliable measurements if contamination changes its physical or chemical behavior. Comparing measured properties with pure-compound expectations helps chemists recognize that problem and prepare materials whose composition and performance remain consistent.