Journal
/
Neuroscience
/
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

DOI:
10.3791/55364-v

•

07:54 min

•

April 13, 2017

•

, , , , , , ,
1Biomedical Sciences & Iowa Center for Advanced Neurotoxicology,Iowa State University

Chapters

  • 00:05Title
  • 00:37Culture of Mixed Glial Cells
  • 02:14Isolation of Microglial Cells
  • 05:31Results: Characteristics of Primary Microglial Cultures
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.

Tags

Keywords: CD11bMagnetic IsolationPrimary MicrogliaPurityVersatilityPostnatalNeuroscienceNeural InflammationRapid IsolationTrypsin EDTACell CultureCell StrainerTriturationCentrifugation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55364?article_type=v

The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism
check_url/55364?article_type=v

Effects of Transcranial Alternating Current Stimulation on the Primary Motor Cortex by Online Combined Approach with Transcranial Magnetic Stimulation
check_url/55364?article_type=v

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
check_url/55364?article_type=v

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium
check_url/55364?article_type=v

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
check_url/55364?article_type=v

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
check_url/55364?article_type=v

Rapid Isolation of Dorsal Root Ganglion Macrophages
check_url/55364?article_type=v

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
check_url/55364?article_type=v

Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
check_url/55364?article_type=v

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Read Article