Volumetric Pipette
Volumetric Pipette - Related Videos
06:35
Introduction to Serological Pipettes and Pipettors
0 Views •
2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:26
Quantitative Assessment Protocol for Facial Soft Tissue Volumetric Changes with Stereophotogrammetry
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2025
We present a detailed protocol for three-dimensional analysis of soft tissue changes with the use of stereophotogrammetry.
05:12
Pressure-polishing Pipettes for Improved Patch-clamp Recording
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Cited by 13 •
2008
This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
FAQs
The liquid level must be judged against the calibration mark without viewing the pipette from above or below. Reading at eye level reduces visual error caused by parallax, while aligning the meniscus with the mark establishes the intended measurement condition. This careful alignment is especially important when preparing solutions for quantitative chemistry, where small volume differences can affect results.
The narrow calibrated bore allows the liquid level to be positioned precisely, while the enlarged central bulb provides space to hold the designated volume. Together, these features support accurate adjustment to the calibration mark and controlled delivery by gravity. The design therefore links precise volume measurement with reproducible transfer during analytical procedures.
Rinsing, filling, meniscus adjustment, and drainage technique all influence the delivered volume. Inadequate rinsing or careless filling can make the liquid condition inconsistent, while an incorrect meniscus reading changes the measured amount. Allowing the liquid to drain appropriately by gravity helps maintain consistent transfers and reduces error across repeated measurements.
Begin by rinsing the pipette appropriately, then fill it with the liquid and adjust the meniscus to the calibration mark while viewing at eye level. After positioning it for transfer, allow the liquid to drain by gravity. Consistent attention to these steps improves the reliability and reproducibility of the measured volume.
A volumetric pipette transfers a precisely defined amount of liquid into the preparation being made. This supports standard solutions, in which a known volume is important, and serial dilutions, where controlled transfers are repeated to obtain progressively diluted solutions. Accurate meniscus adjustment and consistent drainage help preserve the intended relationships between volumes and concentrations.
Titrations and related quantitative analyses depend on reliable volume measurements to support chemical calculations and comparisons. Consistent delivery from a properly handled volumetric pipette helps ensure that the transferred amount matches the calibrated value. As a result, careful technique strengthens the reliability and reproducibility of analytical results rather than allowing avoidable volume errors to influence conclusions.