Endocervical Brush
Endocervical Brush - Related Videos
08:10
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
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Cited by 4 •
2017
We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.
13:57
Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
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Cited by 4 •
2014
The methodology to perform friction force microscopy experiments for contacting brushes is presented: Two polymer brushes that are grafted from (a) substrates and (b) colloidal probes are slid to show that, by using two contacting immiscible brush systems, friction in sliding contacts is reduced compared to miscible brush systems.
06:56
Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
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Cited by 17 •
2013
Poly(ethylene glycol) (PEG) brush-arm star polymers (BASPs) with narrow mass distributions and tunable nanoscopic sizes are synthesized in via ring opening metathesis polymerization (ROMP) of a PEG-norbornene macromonomer followed by transfer of portions of the resulting living brush initiator to vials containing varied amounts of a rigid, photo-cleavable bis-norbornene crosslinker.
12:34
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
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Cited by 24 •
2014
The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.
10:25
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
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Cited by 9 •
2019
Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.
FAQs
Rotation allows the soft bristles to contact the lining of the endocervical canal rather than sampling only the cervical opening. This contact helps gather epithelial cells and secretions from the intended site. Adequate contact and collection are important because the resulting specimen supports laboratory assessment of cervical health and strengthens the reliability of subsequent screening or diagnostic workflows.
The brush collects epithelial cells from the endocervical canal together with associated secretions. These materials provide the specimen analyzed in cervical cytology and can also support human papillomavirus testing. Their value comes from representing the canal lining, where cellular changes or infection-related risk may be assessed through laboratory methods.
After collection, the material from an endocervical brush can be transferred either to a slide or into a liquid-based preservative. Both options move the collected specimen into a form suitable for laboratory analysis, but the overview identifies them as alternative preparation pathways rather than assigning different clinical findings to each. The selected pathway therefore forms part of the laboratory workflow.
A single sampling approach can provide collected cervical material for different laboratory purposes. In cervical cytology, the specimen is examined for abnormal cellular changes; in human papillomavirus testing, it helps evaluate infection-related risk. Using the brush within these workflows connects physical sampling of the endocervical canal with screening or diagnostic assessment of cervical health.
Collection begins by guiding the brush through the cervical opening into the endocervical canal. The brush is then rotated so its bristles contact the canal lining and collect epithelial cells and secretions. Finally, the material is transferred to a slide or liquid-based preservative for laboratory analysis. Each step links the sampling site to the final test specimen.
Specimen adequacy determines whether the collected material sufficiently represents the intended sampling site for laboratory evaluation. Proper contact with the canal lining and effective transfer of the collected material help produce an adequate specimen. In turn, adequate sampling strengthens screening and diagnostic workflows by supporting evaluation for abnormal cellular changes and infection-related risk.
Clinicians may use this sampling approach when cervical material is needed for cervical cytology or human papillomavirus testing. These applications support evaluation of cervical health, including assessment for abnormal cellular changes and infection-related risk. The method is therefore relevant to women’s health screening and to diagnostic workflows that depend on an appropriately collected cervical specimen.