Journal
/
Medicine
/
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

DOI:
10.3791/51906-v

•

12:34 min

•

July 06, 2014

•

, , ,
1Department of Medical Microbiology,University of Manitoba, 2Department of Community Health Sciences,University of Manitoba

Chapters

  • 00:00Title
  • 02:22Isolation of Cervical Mononuclear Cells (CMCs)
  • 05:11CMC Surface Staining and Flow Cytometry
  • 08:06Collection and Preparation of Cervical-Vaginal Lavage (CVL)
  • 08:36Data Acquisition and Analysis
  • 11:56Conclusion

Summary

Automatic Translation

Automatic Translation

The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.

Tags

Keywords: Mucosal ImmunityFemale Genital TractHIV TransmissionSexually Transmitted InfectionsCervical Mononuclear CellsFlow CytometryCervical-vaginal LavageImmune Mediators

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51906?article_type=v

Isolation of Human Islets from Partially Pancreatectomized Patients
check_url/51906?article_type=v

Collection Protocol for Human Pancreas
check_url/51906?article_type=v

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
check_url/51906?article_type=v

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
check_url/51906?article_type=v

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
check_url/51906?article_type=v

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
check_url/51906?article_type=v

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
check_url/51906?article_type=v

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
check_url/51906?article_type=v

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow
check_url/51906?article_type=v

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

Read Article