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06:40
Seven Steps to Stellate Cells
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Cited by 67 •
2011
Here we describe a method for the isolation of hepatic stellate cells from mouse liver. For stellate cell purification, mouse livers are digested in situ and in vitro by pronase-collagenase treatment prior to density gradient centrifugation. This technique yields highly pure hepatic stellate cells.
09:12
Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc
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Cited by 6 •
2009
Here we describe a protocol to examine the migration of glial cells into the developing Drosophila eye using live microscopic analysis paired with GFP tagged glial cells.
03:20
Nuclear Transmutation
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2020
Nuclear transmutation is the conversion of one nuclide into another. It can occur by the radioactive decay of a nucleus, or the reaction of a nucleus with another particle. The first manmade nucleus was produced in Ernest Rutherford’s laboratory in 1919 by a transmutation reaction, the bombardment of one type of nuclei with other nuclei or with neutrons. Rutherford bombarded nitrogen-14 atoms with high-speed α particles from a natural radioactive isotope of radium and observed protons being...
07:58
Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools
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Cited by 10 •
2020
Here, we present a protocol to use a curvelet transform-based, open-source MATLAB software tool for quantifying fibrillar collagen organization in the extracellular matrix of both normal and diseased tissues. This tool can be applied to images with collagen fibers or other types of line-like structures.
09:30
Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
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Cited by 2 •
2023
The protocol summarizes the best practices to minimize microbial bioburden in a cleanroom environment and includes strategies such as environmental monitoring, process monitoring, and product sterility testing. It is relevant for manufacturing and testing facilities that are required to meet current good tissue practice standards and current good manufacturing practice standards.
FAQs
The examiner considers both the location of reproduced pain and the movement restriction observed during the maneuver. Symptoms associated with the hip may support concern for intra-articular hip pathology, whereas posterior pelvic pain may point toward sacroiliac-region involvement. Because the same maneuver can involve both areas, interpretation depends on the overall clinical findings.
Pain and restricted movement are clinically meaningful findings, but neither identifies a single condition by itself. Pain may arise from hip joint or sacroiliac-region dysfunction, while limited movement indicates an abnormal response during the assessed range of motion. The examiner therefore compares the finding with the patient’s history and other examination results.
The alternative name reflects the three principal hip positions used during the maneuver: flexion, abduction, and external rotation. Recognizing both names helps clinicians connect descriptions in examination records or clinical discussions. The two terms refer to the same examination approach rather than separate tests with different purposes.
Patient history supplies context that the maneuver cannot provide on its own. Information about the location and character of symptoms helps determine whether reproduced pain is more consistent with hip involvement or posterior pelvic pain. Combining that history with movement findings and other examination results reduces the risk of treating one observation as a complete conclusion.
The patient lies supine while the examiner positions the tested hip in flexion, abduction, and external rotation, placing the ankle over the opposite knee. Gentle downward pressure is then applied. The examiner observes the available movement and whether this pressure reproduces symptoms, recording pain or restriction for clinical correlation.
Clinicians may use the maneuver when assessing symptoms that could involve the hip joint or sacroiliac region. Its findings can contribute to evaluation of intra-articular hip pathology and posterior pelvic pain. The test is most useful as one part of a broader examination, because its results require comparison with the patient’s history and other findings.