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Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools
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Bioengineering
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JoVE Journal Bioengineering
Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

DOI:
10.3791/61931-v

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07:58 min

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November 11, 2020

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1Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging,University of Wisconsin-Madison, 2Departments of Medical Physics and Biomedical Engineering,University of Wisconsin-Madison, 3Morgridge Institute for Research, Madison, Wisconsin, 4test,test

Chapters

  • 00:04Introduction
  • 00:43CT-FIRE Fiber Extraction and Region of Interest (ROI) Analysis
  • 02:34Advanced CT-FIRE Post-Processing
  • 04:09CurveAlign Fiber Analysis: CT Mode Without Boundary
  • 05:03CurveAlign Fiber Analysis: CT-FIRE Mode with Boundary
  • 06:14CurveAlign Region of Interest (ROI) Analysis
  • 06:56Results: Representative Tumor-Associated Human Breast Cancer Tissue Microarray (TMA) Collagen Signatures
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to use a curvelet transform-based, open-source MATLAB software tool for quantifying fibrillar collagen organization in the extracellular matrix of both normal and diseased tissues. This tool can be applied to images with collagen fibers or other types of line-like structures.

Tags

Keywords: Fibrillar CollagenCollagen OrganizationExtracellular MatrixCurvelet TransformCT-FIREImage AnalysisFiber ExtractionRegion Of InterestPost-processing

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