Borates
Borates - Related Videos
08:55
Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
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Cited by 5 •
2019
Here we present a protocol to express, solubilize, and purify several eukaryotic borate transporters with homology to the SLC4 transporter family using yeast. We also describe a chemical cross-linking assay to assess the purified homomeric proteins for multimeric assembly. These protocols can be adapted for other challenging membrane proteins.
04:17
Quantification of Bacterial Alginate Using the Uronic Acid Carbazole Assay
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2025
Source: Al Ahmar, R., et al. Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J. Vis. Exp. (2020)This video demonstrates a colorimetric assay to quantify alginate produced by Pseudomonas aeruginosa, using carbazole to detect uronic acids after acid hydrolysis. The method enables comparative analysis of alginate production normalized to bacterial biomass in research and clinical studies.
11:05
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
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Cited by 1 •
2016
Free solution capillary electrophoresis is a fast, cheap and robust analytical method that enables the quantitative monitoring of chemical reactions in real time. Its utility for rapid, convenient and precise analysis is demonstrated here through analysis of covalent peptide grafting onto chitosan films for improved cell adhesion.
09:58
Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts
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Cited by 3 •
2015
The goal of this manuscript is to demonstrate a straightforward method for the preparation of ynones from acyl chloride and potassium alkynyltrifluoroborate salt starting materials. The one-pot reaction proceeds rapidly in the presence of boron trichloride without exclusion of air and moisture.
11:18
Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography
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Cited by 48 •
2014
The bacterial cell wall is composed of peptidoglycan, a macromolecular network of sugar strands crosslinked by peptides. Ultra Performance Liquid Chromatography provides high resolution and throughput for novel discoveries of peptidoglycan composition. We present a procedure for the isolation of cell walls (sacculi) and their subsequent preparation for analysis via UPLC.
FAQs
When heated, borate components act as fluxes by lowering melting temperatures and promoting glass formation. This changes how an oxide mixture develops during thermal processing, potentially allowing glass or ceramic constituents to form under less demanding melting conditions. Engineers therefore consider borate content when tailoring processing conditions and the resulting structure of the manufactured material.
Borates influence viscosity by modifying oxide networks rather than serving only as heat-responsive additives. These network changes affect how readily a processed material flows and consolidates, while also influencing durability and resistance to heat or chemical attack. Adjusting the borate contribution can therefore shift the balance between processing behavior and long-term material stability.
Phase behavior helps engineers understand how composition responds during processing and how the material develops its final structure. Examining these changes supports the selection of suitable processing conditions for glass and ceramic systems. It also helps connect composition with properties such as strength, stability, heat resistance, and resistance to chemical attack.
A practical approach begins by selecting a borate composition suited to the intended material, then relating that composition to thermal processing and phase behavior. Engineers can next evaluate how the resulting structure affects viscosity, durability, and resistance to heat or chemical attack. This composition-processing-property relationship guides development of materials with targeted performance.
Borate-containing formulations support several engineering applications, including glass and ceramic manufacturing, enamels, thermal insulation, and cement-based materials. Their usefulness comes from the combined effects of fluxing, glass formation, oxide-network modification, and resistance-related properties. The relevant balance depends on whether the application emphasizes processing, thermal stability, chemical durability, or structural performance.
By modifying oxide networks and influencing phase development, borates can help engineers tailor materials for improved strength, stability, and resistance to heat or chemical attack. These effects are relevant when glass, ceramic, insulation, enamel, or cement-based products must maintain performance under demanding conditions. Composition and processing choices determine which combination of properties is achieved.