Optical Lever Readout

Optical lever readout is a precision measurement technique that converts a small mechanical angular displacement into a larger, measurable movement of a light spot, making it valuable for sensing and instrumentation. In a typical system, a laser reflects from a mirror or cantilever, and a slight change in the reflecting surface redirects the beam; the resulting spot displacement at a position-sensitive detector is proportional to the surface angle and depends on the optical path length. Engineers use this method to measure cantilever deflection, vibration, force, and displacement in atomic force microscopy, microelectromechanical systems, and other high-sensitivity devices.

Optical Lever Readout - Related Videos

Research

JoVE Journal - Engineering

Measurement of Chladni Mode Shapes with an Optical Lever Method

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2020

A simple method of measuring the Chladni mode shape on an elastic plate by the principle of an optical lever is proposed.

Measuring the Interaction Force Between a Droplet and a Super-hydrophobic Substrate by the Optical Lever Method

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2019

The protocol aims to investigate the interaction between droplets and super-hydrophobic substrates in the air. This includes calibrating the measurement system and measuring the interaction force at super-hydrophobic substrates with different grid fractions.

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

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Cited by 5 •

2015

We describe an endpoint digital assay for quantifying nucleic acids with a simplified (analog) readout. We measure bulk fluorescence of droplet-based digital assays using a standard qPCR machine rather than specialized instrumentation and confirm our results by microscopy.

Research

JoVE Journal - Neuroscience
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Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats

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Cited by 18 •

2019

A detailed protocol for the assessment of structural and visual readouts in rodents by optical coherence tomography and optokinetic response is presented. The results provide valuable insights for ophthalmologic as well as neurologic research.

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

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Cited by 2 •

2012

Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.

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