Journal
/
Neuroscience
/
A Molecular Readout of Long-term Olfactory Adaptation in C. elegans
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

DOI:
10.3791/4443-v

•

11:30 min

•

December 22, 2012

•

, , ,
1Department of Biological Sciences and Institute for Neuroscience,George Washington University, 2Fred Hutchinson Cancer Research Center, 3Department of Cell and Tissue Biology,University of California San Francisco

Chapters

  • 00:05Title
  • 01:57Cultivation and Maintenance of Animals for Nuclear Translocation Assays
  • 03:26Long-term Odor Induced Nuclear Translocation Assays
  • 05:44Scoring the Odor-induced Nuclear Translocation Event
  • 08:54Results: Molecular Readout of Long-term Olfactory Adaptation in C. elegans
  • 11:09Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.

Tags

Molecular ReadoutLong-term Olfactory AdaptationC. ElegansSensory NeuronsAdaptation ResponseOlfactory CircuitGenetic ScreenMutantsVolatile OdorsAmphid Wing Cells Type C (AWC) Sensory NeuronsChemotaxis Behavioral AssayShort-term Olfactory AdaptationLong-term Olfactory AdaptationProtein Kinase G (PKG)EGL-4 Protein

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4443?article_type=v

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays
check_url/4443?article_type=v

In vivo Laser Axotomy in C. elegans
check_url/4443?article_type=v

Automated Quantification of Synaptic Fluorescence in C. elegans
check_url/4443?article_type=v

In vivo Neuronal Calcium Imaging in C. elegans
check_url/4443?article_type=v

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish
check_url/4443?article_type=v

Long-term Time Lapse Imaging of Mouse Cochlear Explants
check_url/4443?article_type=v

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
check_url/4443?article_type=v

Automated Analysis of C. elegans Swim Behavior Using CeleST Software
check_url/4443?article_type=v

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
check_url/4443?article_type=v

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

Read Article