Optical Particle Counter
Optical Particle Counter - Related Videos
07:20
Trapping of Micro Particles in Nanoplasmonic Optical Lattice
0 Views •
2017
We describe a procedure to optically trap micro-particles in nanoplasmonic optical lattice.
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
0 Views •
Cited by 56 •
2010
The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
0 Views •
Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
13:39
Optical Trapping of Nanoparticles
0 Views •
Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
13:16
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
0 Views •
Cited by 18 •
2012
The Moxi Z miniature automated cell counter is a novel instrument that combines the Coulter Principle with patented thin-film sensor technology and a proprietary software algorithm to perform sizing and counting of a broad size range of particles as well as to determine the overall health of monodisperse mammalian cell cultures. This protocol describes the use of this instrument for counting and assessing the health of cell cultures.
FAQs
As particles cross the illuminated sensing zone, each produces a light-scattering event that reaches the photodetector as a pulse. The instrument records these pulses as individual particle counts and analyzes their measured signal characteristics to estimate how particles are distributed across different size ranges. This links optical responses with quantitative contamination information.
The sensing zone provides a defined location where particles encounter the focused light source, making their interactions measurable as they pass through. The photodetector then converts the resulting scattered-light events into electrical pulses for analysis. Together, these components establish the measurement pathway from particle passage to recorded counts and estimated size distributions.
A total count indicates how many particles were measured, whereas a size distribution shows how those particles are apportioned among estimated size ranges. That added detail helps engineers characterize the nature of airborne contamination and compare conditions more meaningfully. It can support evaluations of air cleanliness, filtration performance, and possible process problems.
The workflow begins by directing a laser or another focused light source into a sensing zone. Suspended particles pass through that zone and scatter the incident light. A photodetector captures each resulting pulse, after which the instrument compiles particle counts and estimated size distributions. Engineers can then interpret the measurements for the environment or system being assessed.
Engineers apply these instruments when they need quantitative information about airborne contamination or air cleanliness. Supported uses include cleanroom certification, indoor air-quality assessment, industrial air-quality assessment, filter performance testing, and contamination control. The selected application determines whether the measurements primarily document environmental conditions, evaluate a filtration system, or help investigate a process concern.
Measurements provide evidence for evaluating whether a filtration system is controlling suspended-particle levels as intended. They also help identify process problems by showing changes in particle counts or estimated size distributions. In engineering programs, these results contribute to contamination-control activities and help maintain environmental standards in cleanroom, indoor, and industrial settings.