Eps Extraction
Eps Extraction - Related Videos
02:50
An Assay to Study Bile-Salt Induced Biofilm Formation through EPS Matrix Detection
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2025
This video demonstrates a fluorescence-based method for quantifying bile salt-induced biofilm formation. Upon the addition of bile salts in the culture of a pathogenic enteric bacteria, biofilm production is detected using a fluorescent lectin that binds to the polysaccharides in the extracellular polymeric substance (EPS) matrix of the biofilm.
01:21
EPS and iPS Cells in Disease Research
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2023
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
06:10
Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge
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Cited by 201 •
2016
The protocol provides a methodology to solubilize aerobic granular sludge in order to extract alginate-like extracellular polymers (ALE).
02:55
Extraction - Concepts
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2020
Extraction Extraction is a common technique used in organic chemistry to isolate a target compound. In the extraction process, a solute is transferred from one phase to another to separate it from unreacted starting materials or impurities. Extraction is also used to facilitate the isolation of a solute from a reaction solvent that is difficult to remove by evaporation, such as a solvent with a high boiling point. Generally, there are three types of extractions. First, in solid-liquid...
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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Cited by 10 •
2021
Here, we present an optimized on-filter digestion protocol with detailed information about the following: protein digestion, peptide purification and data independent acquisition analysis. This strategy is applied to the analysis of expressed prostatic secretions-urine samples and allows high proteome coverage and low missing value label-free profiling of the urinary proteome.
FAQs
The recovered fraction depends on how strongly polymer associations are loosened during processing. Soluble material can be collected after it is released into the surrounding liquid, whereas more tightly associated material requires conditions that disrupt its attachment to cells or matrix structures. Separating these fractions helps researchers compare how EPS is organized and how strongly it remains associated with microbial communities.
Extraction strength requires a balance between recovery and preservation. Conditions that are too mild may leave matrix material attached to cells, while overly strong treatment can promote cell disruption or chemically alter the recovered polymers. Controlling this variable improves confidence that measured polysaccharides, proteins, nucleic acids, and other components represent the original environmental matrix rather than extraction-induced changes.
Polysaccharides, proteins, nucleic acids, and other recovered components contribute differently to matrix behavior. Together, they can influence microbial adhesion, aggregation, contaminant binding, and biofilm stability. Examining their composition therefore connects laboratory measurements with the physical and chemical properties of biofilms, flocs, and sediments, including how these materials form, persist, and interact with pollutants.
A typical workflow begins with a biofilm, floc, or sediment sample, followed by treatment that loosens cell–polymer associations. The released material is then collected, with soluble and bound fractions distinguished when appropriate. Throughout the process, extraction strength is controlled to reduce cell disruption and chemical alteration, preserving the matrix characteristics needed for later characterization.
Extracts provide material for characterizing the composition of the extracellular matrix, including its polysaccharides, proteins, nucleic acids, and other constituents. Comparing the recovered material and fractions can clarify which components are associated with cells or remain soluble. These results support interpretation of matrix properties linked to adhesion, aggregation, contaminant binding, and biofilm stability.
The method is useful when researchers need to connect microbial matrix composition with environmental processes. In wastewater treatment and sludge studies, it can support investigations of floc behavior and settling. In aquatic and soil systems, the extracts help examine microbial ecology and the fate and transport of pollutants, especially where polymer matrices influence contaminant binding or biofilm structure.