Olfactory Nerve Recording
Olfactory Nerve Recording - Related Videos
07:02
Extracellular Multi-Unit Recording from the Olfactory Nerve of Teleosts
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Cited by 6 •
2020
Extracellular multi-unit recording from the olfactory nerve is a sensitive, robust and reproducible method for assessing olfactory sensitivity in marine fish. It records primary sensory input and is independent of external salinity.
10:33
Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury
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Cited by 16 •
2014
Olfactory ensheathing cells (OECs) are neural crest cells which allow growth of the primary olfactory neurons. This specific property can be used for cellular transplantation. We present here a model of cellular transplantation based on the use of OECs in a laryngeal nerve injury model.
11:08
Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis
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Cited by 3 •
2016
Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.
06:22
Perforated Patch-Clamp Recording of Olfactory Sensory Neurons in Mice
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2025
This video demonstrates electrophysiological recording of olfactory sensory neurons (OSNs) in mice using a perforated patch-clamp technique. The olfactory epithelium, containing OSNs with fluorophore-tagged odor receptors, is isolated from a mouse and secured in a recording chamber. A recording pipette is clamped to the dendritic knob of an OSN, and nystatin within the pipette perforates the membrane to form electrical connectivity with the cell. Finally, another pipette is used to deliver an...
08:08
Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
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Cited by 13 •
2012
Olfactory receptor neurons (ORNs) convert odor signals first into a receptor current that in turn triggers action potentials that are conveyed to second order neurons in the olfactory bulb. Here we describe the suction pipette technique to record simultaneously the odorant-induced receptor current and action potentials from mouse ORNs.
FAQs
Odorant molecules first bind to receptors in the olfactory epithelium. That binding activates intracellular transduction pathways, which alter the membrane potential of olfactory receptor neurons. The resulting electrical changes are transmitted through the olfactory nerve, creating signals that electrodes can capture and analyze as a physiological response to airborne chemicals.
The recorded voltage change provides a direct physiological readout of how olfactory receptor neurons respond when they detect an airborne chemical. It therefore links chemical detection at the receptor level with neural signaling. Analysis of these electrical responses can show that an odorant has activated the olfactory system, rather than merely indicating that a chemical is present in the environment.
The olfactory epithelium is the site where odorant molecules interact with receptor neurons, while the olfactory nerve carries the resulting signals onward. Recording from this pathway connects the initial environmental stimulus with its neural representation. That connection is important for studying how organisms detect changing airborne conditions and how chemical cues are converted into biological information.
Electrodes capture voltage changes generated as olfactory receptor neurons respond to odorant-triggered transduction. The recorded electrical activity can then be analyzed to characterize the response of the olfactory system to airborne chemicals. In environmental studies, this provides a measurement based on biological detection, complementing investigations of pollutants, volatile organic compounds, and other airborne cues.
By measuring neural responses to airborne chemicals, the technique can help characterize how organisms detect pollutants and volatile organic compounds. These recordings provide physiological information about odor sensing that can support chemical sensing research and exposure assessment. The approach is especially relevant when researchers want to examine the biological response associated with chemicals present in changing environments.
Olfactory nerve recordings can help investigate whether airborne pollutants, volatile organic compounds, or other environmental cues produce detectable responses in the olfactory system. The resulting signals support studies of chemical sensing and exposure assessment, while also helping researchers examine how organisms respond physiologically as the chemical conditions of their surroundings change.