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JoVE Journal
Neuroscience

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Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis
 

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

Article DOI: 10.3791/54108 11:08 min June 3rd, 2016
June 3rd, 2016

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Summary

Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.

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Tags

Temperature-induced Neuronal Activity Calcium Changes Olfactory Bulb Xenopus Laevis Nasal Epithelium Affections Calcium Imaging Cell Electroporation Bolus-loading Activity Correlation Imaging Tadpole Gel Cushion Stereo Microscope Dye Crystal Cathode Anode
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