Plga Particle Synthesis
Plga Particle Synthesis - Related Videos
12:48
PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
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Cited by 211 •
2013
We describe the production and characterization of nanoparticles and microparticles composed of poly(lactic-co-glycolic acid) using vitamin E-TPGS as an emulsifier. By varying formulation parameters such as the concentration of emulsifier, it is possible to produce nanoparticles with mean diameters ranging from 220 nm to 1.98 µm.
09:06
Intra-lymph Node Injection of Biodegradable Polymer Particles
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Cited by 45 •
2014
Lymph nodes are the immunological tissues that orchestrate immune response and are a critical target for vaccines. Biomaterials have been employed to better target lymph nodes and to control delivery of antigens or adjuvants. This paper describes a technique combining these ideas to inject biocompatible polymer particles into lymph nodes.
09:34
Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
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Cited by 12 •
2017
Here, we present a protocol to visualize blood vessel formation in vivo and in real-time in 3D scaffolds by multiphoton microscopy. Angiogenesis in genetically modified scaffolds was studied in a murine calvarial critical bone defect model. More new blood vessels were detected in the treatment group than in controls.
06:56
Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
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Cited by 17 •
2013
Poly(ethylene glycol) (PEG) brush-arm star polymers (BASPs) with narrow mass distributions and tunable nanoscopic sizes are synthesized in via ring opening metathesis polymerization (ROMP) of a PEG-norbornene macromonomer followed by transfer of portions of the resulting living brush initiator to vials containing varied amounts of a rigid, photo-cleavable bis-norbornene crosslinker.
06:48
Synthesis and Characterization of Self-Assembled Metal-Organic Framework Monolayers Using Polymer-Coated Particles
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2024
A protocol for the synthesis and characterization of self-assembled metal-organic framework monolayers is provided using polymer-grafted, metal-organic framework (MOF) crystals. The procedure shows that polymer-grafted MOF particles can be self-assembled at an air-water interface resulting in well-formed, free-standing, monolayer structures as evidenced by scanning electron microscopy imaging.
FAQs
After the polymer and target compound are dissolved in an organic solvent, the mixture is dispersed into an aqueous surfactant phase, forming droplets. Removing the organic solvent causes the dissolved PLGA to solidify around the incorporated compound. This transition converts liquid droplets into discrete particles and establishes the physical basis for encapsulation and subsequent controlled release.
Polymer concentration, surfactant level, mixing rate, and the ratio between phases all affect particle formation. These variables influence how droplets form and stabilize before solvent removal, thereby changing particle size and morphology. Adjusting them also affects compound loading and release behavior, so formulation conditions must be selected according to the intended delivery outcome.
The aqueous surfactant phase helps stabilize the dispersed organic droplets, while the mixing rate influences how those droplets form during dispersion. Changes in either condition can alter the resulting particle dimensions and structure. Because size and morphology are linked to loading and release behavior, these parameters are important controls rather than incidental processing details.
Encapsulating an active compound within PLGA particles places the compound inside a polymer-based delivery structure rather than leaving it freely dispersed. The resulting particle characteristics, including size, morphology, and loading, influence release behavior. This makes synthesis conditions relevant to designing delivery systems that regulate how an agent becomes available under the intended application conditions.
First, dissolve PLGA and the target compound in an organic solvent. Next, disperse that solution into an aqueous phase containing surfactant to create droplets. Finally, remove the organic solvent so the droplets solidify into particles. The selected polymer concentration, phase ratio, mixing rate, and surfactant level should remain controlled throughout the workflow.
The workflow requires PLGA, the compound being delivered, an organic solvent, an aqueous phase, and a surfactant. It also requires controlled mixing and solvent removal. These components and conditions work together to form and stabilize droplets, then solidify them. Their adjustment determines measurable outcomes such as particle size, morphology, loading, and release behavior.
Environmental researchers may apply the technique when they need controlled delivery of agrochemicals or other functional agents. Encapsulation can help regulate exposure and improve material efficiency compared with uncontrolled release into surrounding ecosystems. The approach is therefore relevant to environmental delivery strategies in which the timing and extent of an agent's availability are important research considerations.
Evaluation should focus on particle size, morphology, compound loading, and release behavior because these properties indicate how the formulation may perform as a delivery system. In environmental applications, researchers can relate those outcomes to exposure regulation, material efficiency, and the potential to limit uncontrolled release. The results help connect processing conditions with ecological delivery objectives.