Time Slicing

Time slicing is the practice of dividing a continuous time record into defined intervals to examine how a system changes over time. In environmental research, investigators assign observations such as temperature, precipitation, pollutant levels, or land cover to consistent time windows, then compare patterns within and between slices. This approach simplifies complex temporal datasets, helps reveal trends and shifts that may be obscured in long-term averages, and supports analysis of seasonal variation, environmental change, and human impacts. Time slicing is useful for organizing monitoring data, evaluating conditions before and after an event, and informing environmental assessment and management.

Time Slicing - Related Videos

Research

JoVE EoE - Neuroimaging

Real-Time Gene Expression Monitoring in Brain Slices via Three-Dimensional Imaging

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2025

Source: Shimojo, H., et al. Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis. J. Vis. Exp. (2019)In this video, cortical slice cultures from transgenic mouse embryos are used to monitor gene expression through Three-dimensional imaging. Bioluminescence, fluorescence, and bright-field images are used to visualize real-time gene expression during neuronal differentiation within the native tissue.

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

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Cited by 10 •

2012

We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

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Cited by 6 •

2010

This protocol describes an organotypic slice assay optimized for the postnatal brain and high-resolution time-lapse imaging of neuroblast migration in the rostral migratory stream.

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

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Cited by 15 •

2013

Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.

Time-Lapse Imaging of Cortical Neuron Radial Migration in Transduced Mouse Embryonic Brain Slices

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2025

Source: Wiegreffe, C., et. al., Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation. J. Vis. Exp. (2017) This video demonstrates time-lapse confocal microscopy to observe cortical neuron radial migration from the subventricular zone to the cortical plate via radial glial scaffolds in transduced mouse embryonic brain slices.

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