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Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
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Neuroscience
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JoVE Journal Neuroscience
Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

DOI:
10.3791/4061-v

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10:25 min

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September 12, 2012

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,
1The Cellular Neurobiology Unit,Centre de Recherche Université Laval Robert-Giffard

Chapters

  • 00:05Title
  • 01:18Acute Slices Preparation
  • 04:25Time-lapse Imaging of Neuroblast Migration
  • 05:50Analyzing Neuroblast Migration
  • 08:46Results: Time-lapse Imaging of Neuroblast Migration in Acute Slice
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.

Tags

Time-lapse ImagingNeuroblast MigrationAdult Mouse ForebrainNeural Stem CellsSubventricular Zone (SVZ)Rostral Migratory Stream (RMS)Olfactory Bulb (OB)Astrocytic ProcessesBlood VesselsCell MigrationAcute SlicesRodent BrainIn Vivo ImagingDIC OpticsFluorescent ProteinsViral Labeling

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