Base Editing
Base Editing - Related Videos
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
02:23
RNA Editing
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2020
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
09:56
Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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Cited by 1 •
2025
The efficiency of the Improved Genome Editing via Oviductal Nucleic Acids Delivery (I-GONAD) method is comparable to traditional microinjection, which requires zygote collection from donor females and transfer to pseudo-pregnant females. This protocol demonstrates its effectiveness by introducing CRISPR/Cas9-induced mutations into the ROSA26 locus on chromosome 6.
08:50
A Nonsequencing Approach for the Rapid Detection of RNA Editing
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2022
Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.
FAQs
The catalytically impaired CRISPR-Cas protein provides sequence recognition and positions the editing machinery at a selected DNA site. The attached nucleotide deaminase then performs the chemical conversion within a limited region called the editing window. This division of roles enables sequence-directed nucleotide changes while avoiding the DNA-cleavage activity associated with a conventional double-strand break.
A double-strand break can introduce unwanted DNA damage during genome editing. Base Editing approaches nucleotide conversion without creating that type of break, which can support more focused correction of single-nucleotide variants. This distinction is especially relevant when researchers want to examine the effect of one genetic change while reducing damage associated with cutting both DNA strands.
The deaminase acts only within a defined editing window, so the target nucleotide must occur in a compatible position for conversion. Other editable nucleotides located in the same window may also undergo bystander changes. Consequently, researchers must consider both the intended nucleotide and nearby sequence changes when evaluating editing precision and experimental results.
In genetics research, Base Editing can help test how individual nucleotide variants affect gene function. Researchers can use targeted corrections or introduce relevant sequence changes to support functional studies and disease modeling. Comparing outcomes between edited and unedited genetic backgrounds can clarify whether a specific variant contributes to an observed biological or disease-related phenotype.
The technique supports investigations of gene regulation as well as mutation function and disease mechanisms. By directing a defined nucleotide change to a selected genomic sequence, researchers can examine how sequence variation influences gene activity. These applications extend its value from correcting candidate variants to testing genetic explanations for differences in cellular or organismal outcomes.
Therapeutic development must account for restricted editing windows, unintended bystander changes, and activity at off-target sites. These factors can affect whether the desired nucleotide is changed selectively enough for a clinical application. Although avoiding double-strand breaks may reduce unwanted DNA damage, it does not eliminate the need to evaluate editing specificity and the consequences of unintended changes.