Nematode Synchronization
Nematode Synchronization - Related Videos
03:43
Nematode Synchronization: A Method to Obtain Populations of Worms in Identical Stages of Development
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2023
In this video, we show a method of producing a developmentally synchronized population of sterile adult C. elegans. The example protocol synchronizes worms to study oxygen consumption rates in live animals.
09:02
AC Synchronous Machine Synchronization
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2023
Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. Three-phase wound-rotor synchronous generators are the main source of electrical power worldwide. They require a prime mover and an exciter in order to generate power. The prime mover can be a turbine spun by fluid (gas or liquid), thus the sources of the fluid can be water running off a dam through a long nozzle, steam from water evaporated using burned coal, etc. Most power plants including coal,...
03:39
An In Vivo Assay to Detect Mitophagy in Transgenic Nematodes
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2025
This video demonstrates an in vivo assay to study mitophagy in Caenorhabditis elegans. Upon exposing the nematodes to a mitophagy-inducing drug, the occurrence of mitophagy is assessed by co-localization between DCT-1, a mitophagy receptor on the damaged mitochondria, and the autophagosomal membrane protein LGG-1.
11:19
AC Synchronous Machine Characterization
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2023
Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. Three-phase wound-rotor synchronous motors are less popular than permanent magnet rotor synchronous motors due to the brushes required for the rotor field. Synchronous generators are much more common and available in most existing power plants, as they have excellent frequency and voltage regulation. Synchronous motors have the advantage of almost 0% speed regulation due to the fact that the rotor...
09:02
Visualizing Bacteria in Nematodes using Fluorescent Microscopy
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Cited by 20 •
2012
To study the mutualism between Xenorhabdus bacteria and Steinernema nematodes, methods were developed to monitor bacterial presence and location within nematodes. The experimental approach, which can be applied to other systems, entails engineering bacteria to express the green fluorescent protein and visualizing, using fluorescence microscopy bacteria within the transparent nematode.
FAQs
After embryos hatch together, the absence of food causes the larvae to arrest at the first developmental stage. This creates a common biological starting point before experimental feeding begins. Because development is held at the same point, researchers can initiate treatments or observations with less variation caused by differences in developmental timing.
Alkaline hypochlorite treatment dissolves adult nematodes and releases their embryos for collection. The released embryos provide a more uniform starting population than a mixed culture containing adults, embryos, and larvae. In genetic experiments, this separation helps establish populations whose later phenotypes can be compared from the same initial developmental stage.
Synchronization reduces age-related variation that could otherwise obscure genetic effects. Mutant, transgenic, and control strains can be examined after beginning from comparable developmental states, making differences in gene function, development, behavior, or reproduction easier to attribute to genotype rather than unequal ages. The approach therefore strengthens reproducibility in phenotype-based genetic analyses.
Researchers first expose adult animals to an alkaline hypochlorite solution so the adults dissolve and embryos are released. They then collect the embryos and allow them to hatch together without food. The resulting first-stage larvae remain arrested until feeding begins, providing a controlled point from which experiments can be started.
Feeding should be used as the experimental transition out of the first-stage arrest. Before feeding, larvae remain held at a common starting point; once feeding begins, development can proceed for subsequent measurements. Treating feeding as a defined time point helps align observations across populations and supports consistent comparisons of developmental or genetic phenotypes.
Synchronized populations support measurements of gene function, development, behavior, reproduction, and responses to environmental conditions. They are especially useful when comparing mutant, transgenic, and control strains, because the groups begin from comparable developmental states. This controlled design improves interpretation of phenotypic differences and makes results more reproducible across experiments.