Acridine Orange
Acridine Orange - Related Videos
04:18
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
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Cited by 5 •
2019
Here, we present a protocol to use thiazole orange for the detection of DNA in gel electrophoresis experiments. The use of thiazole orange allows elimination of ethidium bromide, and fluorescence detection can be achieved with either UV or blue light.
10:00
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
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Cited by 12 •
2014
Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.
10:10
Exploring the Sequential Cellular Events of Phagocytosis Triggered by Godanti Bhasma in Mammalian Cells
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Cited by 1 •
2025
This study elucidates phagocytic processing of Godanti Bhasma (GB) particles in mammalian cells, characterizing their cellular uptake, vacuole dynamics, acidification, and degradation. These findings not only advance our understanding of fundamental phagocytosis mechanisms but also establish GB as a promising model system for developing novel therapeutic strategies.
10:19
Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
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Cited by 17 •
2011
Described here are protocols used to visualize the dynamic process of MG53-mediated cell membrane repair in whole animals and at the cellular level. These methods can be applied to investigate the cell biology of plasma membrane resealing and regenerative medicine.
04:25
Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO2
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2025
This video demonstrates a quantification technique for CO2 released during substrate oxidation using 14C-radiolabeled substrates. The released 14CO2 is trapped in an alkaline solution and quantified by a scintillation counter. The oxidation of different substrates varies between tissues and reflects the pathophysiological condition of the tissue.
FAQs
After entering biological material, Acridine Orange associates with nucleic acids by intercalating between their bases. The resulting fluorescence depends on whether the bound material is double-stranded DNA, RNA, or single-stranded nucleic acid. This structure-dependent shift creates predominantly green signals for double-stranded DNA and orange-red signals for RNA or single-stranded nucleic acids.
The contrasting colors allow researchers to distinguish nucleic-acid-containing structures according to the type and organization of nucleic acid present. Green fluorescence can emphasize double-stranded DNA, whereas orange-red fluorescence highlights RNA or single-stranded nucleic acids. This distinction supports microscopic examination of cellular organization and helps characterize nucleic-acid patterns in cells and microorganisms.
Fluorescence patterns can be evaluated together with cellular morphology to identify changes in the appearance and organization of stained material. In immunology and infection studies, this combined view supports assessment of cellular viability and morphology rather than relying only on a single color signal. The approach therefore connects nucleic-acid visualization with broader observations of cell condition.
Interpretation should consider both fluorescence color and the morphology of the labeled cells or microorganisms. Predominantly green and orange-red signals provide information about associated nucleic-acid types or structures, while cellular appearance adds contextual evidence about organization, viability, and infection-related changes. Examining these features together makes microscopy findings more informative than evaluating fluorescence alone.
Acridine Orange staining can help visualize nucleic-acid-containing host cells and microorganisms during microscopy-based investigations. Researchers can use the resulting fluorescence and morphological information to examine cellular organization in infection-related samples. This makes the stain relevant to studies of host-pathogen interactions, where cellular and microbial features need to be viewed within the same microscopy-oriented analysis.
Within infection research, Acridine Orange provides a way to visualize nucleic-acid-containing material associated with cells and microorganisms. This supports microscopy-based examination of intracellular organisms and their relationship to host-cell morphology. By combining fluorescence signals with structural observations, researchers can investigate how intracellular infection appears within cellular environments without limiting analysis to the microorganism alone.
In immunology, Acridine Orange staining can contribute to microscopy-based assessment of immune-cell morphology, nucleic-acid organization, and changes associated with cellular viability. Its contrasting fluorescence signals help reveal labeled cellular material, while morphology provides additional context for interpreting cell responses. The technique is therefore useful when immune-cell changes must be examined alongside infection-related cellular features.