Borrelia Burgdorferi
Borrelia Burgdorferi - Related Videos
04:30
Phage Purification from Borrelia burgdorferi
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2026
Source: Eggers, C. H. Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi. J. Vis. Exp. (2022).This video demonstrates the isolation of borrelial phage particles from a Borrelia burgdorferi suspension using polyethylene glycol-mediated phase separation. The sample is centrifuged, treated with a salt solution, and a molecular crowding agent to precipitate phages. Chloroform extraction is done to remove debris and concentrate phage particles. The purified phage...
03:17
Preparation and Induction of Borrelia burgdorferi Cultures for Viral Particle Production
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2025
Source: Eggers, C. H. Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi. J. Vis. Exp. (2022)This video demonstrates the preparation of a genetically modified Borrelia burgdorferi culture engineered with dormant bacteriophage DNA carrying an antibiotic resistance gene. Following antibiotic selection and incubation, a chemical inducer triggers DNA damage, leading to excision, replication, and assembly of new virions containing the resistance gene. After a...
09:01
Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
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Cited by 2 •
2022
The ability of bacteriophage to move DNA between bacterial cells makes them effective tools for the genetic manipulation of their bacterial hosts. Presented here is a methodology for inducing, recovering, and using φBB-1, a bacteriophage of Borrelia burgdorferi, to transduce heterologous DNA between different strains of the Lyme disease spirochete.
10:37
Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
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Cited by 8 •
2022
In vitro culture is a direct detection method for the presence of living bacteria. This protocol describes methods for the culture of diverse Borrelia spirochetes, including those of the Borrelia burgdorferi sensu lato complex, relapsing fever Borrelia species, and Borrelia miyamotoi. These species are fastidious and slow growing but can be cultured.
07:15
Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays
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Cited by 1 •
2022
In vitro transcription assays can decipher the mechanisms of transcriptional regulation in Borreliella burgdorferi. This protocol describes the steps to purify B. burgdorferi RNA polymerase and perform in vitro transcription reactions. Experimental approaches using in vitro transcription assays require reliable purification and storage of active RNA polymerase.
FAQs
Its periplasmic flagella support the spiral-shaped bacterium’s motility. That movement helps it travel through skin and connective tissues after transmission, making motility a central feature for studying infection within the host. In immunology and infection research, this mechanism connects bacterial structure with tissue access and the host response.
Changing which proteins appear on its surface can alter how the bacterium interacts with its surroundings during infection. This surface remodeling is studied as an immune-evasion mechanism because it may help B. burgdorferi persist despite host immune responses. It also provides a mechanistic basis for investigating bacterial adaptation and infection-related immune interactions.
Complement is part of the host immune response that B. burgdorferi can interact with during infection. Examining that interaction helps investigators determine how the bacterium persists in the presence of immune defenses rather than being viewed only as a passive target. This work contributes to broader analyses of immune evasion and host-pathogen relationships in Lyme disease.
These manifestations show that infection can be associated with inflammatory effects in different clinical contexts. Studying Lyme arthritis and neuroborreliosis helps connect the behavior of B. burgdorferi with host inflammatory responses, while also informing diagnostic interpretation and disease-focused research. Their inclusion broadens investigation beyond transmission to the consequences of infection in affected tissues.
Investigators use knowledge of the bacterium’s surface-expressed proteins, complement interactions, and disease-associated manifestations to guide the development and interpretation of diagnostic tests. Understanding these features places test findings within the biology of infection and host response. Diagnostic research therefore links microbiology with immunology and can address challenges in recognizing Lyme disease-related infection.
Following transmission by Ixodes ticks allows researchers to examine infection at the point where the bacterium enters its host and interacts with tissues and immune defenses. This perspective supports investigations of pathogen transmission, including how motility, surface-expressed proteins, and immune evasion relate to persistence. It also provides context for developing disease-prevention strategies.
Research on B. burgdorferi supports several complementary goals: investigating antimicrobial treatment, interpreting disease-related immune responses, and reducing future infection. Treatment studies focus on the organism as an infectious target, whereas prevention research considers transmission by Ixodes ticks and ways to limit risk. Together, these approaches connect laboratory mechanisms with practical strategies for managing and preventing Lyme disease.