Cardiac Ecm Isolation
Cardiac Ecm Isolation - Related Videos
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
12:15
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
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Cited by 35 •
2009
Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.
06:22
Contractility Measurements on Isolated Papillary Muscles for the Investigation of Cardiac Inotropy in Mice
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Cited by 14 •
2015
Murine left ventricular papillary muscle can be used to investigate cardiac contractility in vitro. This article describes in detail the isolation and experimental protocols to study cardiac contractile characteristics.
11:38
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
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Cited by 36 •
2010
Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.
16:33
ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
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Cited by 17 •
2014
A method to obtain nanofibers and complex nanostructures from single or multiple extracellular matrix proteins is described. This method uses protein-surface interactions to create free-standing protein-based materials with tunable composition and architecture for use in a variety of tissue engineering and biotechnology applications.
FAQs
Preserving collagen, elastin, glycoproteins, and proteoglycans is important because these components contribute to the matrix’s structural and biochemical properties. Their retention helps maintain the tissue-derived signals that can influence immune-cell adhesion, migration, and activation. The resulting preparation is therefore more useful for studying matrix biology than a sample in which key components have been lost.
Removing cellular material reduces contributions from the original heart cells and creates a more defined matrix model. This separation helps investigators examine how extracellular signals affect immune-cell behavior, rather than treating responses as the combined effect of cells and matrix. Such control is particularly useful when comparing matrix-associated effects during inflammation or cardiac injury.
Cardiac ECM can provide structural and biochemical signals that shape how immune cells interact with cardiac tissue. These signals may affect whether cells attach to the matrix, move across it, or become activated after contact. Studying these responses with isolated material helps connect matrix properties to immune behavior in a controlled cardiac-tissue context.
The process begins with controlled processing of heart tissue, followed by removal of cellular material while retaining the extracellular matrix. The preparation can then be examined for structural, biochemical, and biological properties. Maintaining this balance is central to producing material that remains representative of cardiac ECM and suitable for downstream immunology, infection, or repair studies.
An isolated preparation can reveal structural features, biochemical composition, and biological activity associated with cardiac ECM. Researchers can use these properties to evaluate how the matrix interacts with immune cells or changes under disease-related conditions. The material therefore supports analysis of both the matrix itself and the responses it elicits in experimental systems.
In immunology and infection research, isolated cardiac ECM provides a defined setting for examining tissue changes linked to inflammation or pathogen-associated injury. Investigators can study how altered matrix structure affects immune-cell adhesion, migration, or activation. This approach helps relate disease-associated tissue remodeling to cellular immune responses without relying only on intact heart tissue.
Because the preparation retains cardiac matrix components and tissue-associated properties, it can inform the design of biomaterials intended for cardiac applications. Researchers can also investigate how matrix characteristics relate to repair processes. These studies connect basic analysis of cardiac ECM with efforts to develop materials or strategies that support restoration after cardiac damage.