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Biology

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Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
 

Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging

Article doi: 10.3791/1433
September 17th, 2009

Summary September 17th, 2009

Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.

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