Cd11b
Cd11b - Related Videos
07:54
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
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Cited by 22 •
2017
Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.
04:45
Magnetic Isolation of Microglia from Mouse Pup Brains
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2025
In this video, we describe a protocol for isolating CD11b cells, including microglia, from mouse pup brains using magnetic-activated cell sorting. The brain tissues are homogenized to obtain single-cell suspensions, which are incubated with anti-CD11b antibody-coated microbeads, followed by a magnetic column-based separation to purify the CD11b+ cell population.
04:34
Measuring Microglial Uptake of Intravitreally-Delivered Fluorescent Particles Using Flow Cytometry
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2025
This video demonstrates flow cytometry for measuring microglial uptake of fluorescent bioparticles after intravitreal delivery in a mouse eye. Flow cytometry detects live cells expressing CD11b and distinguishes phagocytic microglia cells exhibiting green fluorescence from other myeloid cells, including Ly6C-expressing monocytes and Ly6G-expressing neutrophils.
04:09
Isolating Microglia from Mouse Brain Demyelinating Lesions Using Magnetic Activated Cell Sorting
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2025
This video demonstrates a protocol for extracting microglial cells from demyelinating lesions in mouse brains using magnetic cell sorting. The protocol involves dissecting the lesioned brain tissue containing microglial cells, treating the tissue with specific enzymes to obtain a single-cell suspension, and utilizing microglial receptor-specific magnetic beads to isolate the microglial cells.
04:39
In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors
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2025
This video demonstrates an in vitro assay to generate plasmacytoid dendritic cells (pDCs) from common lymphoid progenitor cells (CLPs). Upon co-culturing purified CLPs with γ-irradiated feeder cells in the presence of Fms-like tyrosine kinase-3 ligand (FL), the CLPs differentiate into non-adherent pDCs, the presence of which is confirmed using flow cytometry.
FAQs
CD11b pairs with CD18 to create the integrin complex CR3. This partnership gives leukocytes a receptor capable of binding particles marked by complement, allowing recognition to become a cellular response. The resulting interaction supports firm attachment, promotes movement through inflamed tissue, and contributes to phagocytosis, linking complement activity with physical pathogen clearance.
Complement opsonization marks a particle for recognition by CR3 on leukocytes. When CD11b-containing CR3 binds the complement coating, the cell can attach more firmly and initiate responses associated with engulfment. This mechanism helps connect extracellular immune tagging with phagocytic clearance, making complement recognition relevant to host defense against infectious targets.
Signals generated through CD11b-containing integrin complexes affect the leukocyte cytoskeleton, the internal structure that supports changes in cell shape and movement. These signals help convert receptor binding into firm adhesion and migration while also supporting phagocytosis. Consequently, CD11b contributes not only to particle recognition but also to the physical behaviors required during inflammation.
Its presence on neutrophils, monocytes, macrophages, and other myeloid cells places the receptor across multiple stages of innate immune activity. These cells can use CD11b-related recognition and adhesion functions during surveillance and pathogen clearance, while measurements of the receptor can help examine how different myeloid populations participate in inflammatory responses.
Measuring CD11b can help characterize leukocyte activation and identify how myeloid cells respond during immune challenges. In immunology and infection studies, the measurement can be related to complement-dependent recognition, adhesion, migration, or phagocytosis. It therefore provides a way to investigate cellular behavior in host-pathogen interactions without treating activation as a single, uniform process.
Manipulating CD11b is useful when researchers want to examine how leukocyte recognition and adhesion affect downstream immune activity. Such studies can probe pathogen clearance, inflammatory regulation, and the cellular mechanisms associated with phagocytosis. The approach also supports investigation of inflammatory and immunodeficiency disorders in which innate immune surveillance or clearance may be altered.