Cd34+ Stem Cells
Cd34+ Stem Cells - Related Videos
08:14
Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice
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Cited by 1 •
2023
This study has established a stable and efficient method for the isolation, culture, and functional determination of vascular wall-resident CD34+ stem cells (CD34+ VW-SCs). This easy-to-follow and time-effective isolation method can be utilized by other investigators to study the potential mechanisms involved in cardiovascular diseases.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
06:51
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Cited by 23 •
2016
An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
20:38
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
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Cited by 31 •
2007
10:25
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
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Cited by 7 •
2019
Here, we present a protocol for immunophenotypic characterization and cytokine induced differentiation of cord blood derived CD34+ hematopoietic stem and progenitor cells to the four myeloid lineages. The applications of this protocol include investigations on the effect of myeloid disease mutations or small molecules on myeloid differentiation of the CD34+ cells.
FAQs
Self-renewal maintains a continuing pool of CD34+ stem cells while also allowing production of progenitors that enter blood-cell differentiation pathways. This balance links long-term preservation of the stem-cell population with ongoing hematopoiesis, the process of forming blood cells. Studying that balance helps researchers evaluate how blood formation is maintained or disrupted during infection and treatment.
Differentiation into lymphoid and myeloid lineages connects these cells to the production of leukocytes involved in host defense. Examining this output allows researchers to investigate how changes in hematopoiesis may influence immune-system development and responses to infection. It also provides a framework for studying how pathogens affect the generation of blood and immune cells.
CD34 expression provides a surface-based feature that researchers can use to identify, isolate, and enrich the relevant stem and progenitor-cell population. This makes it possible to examine their function and responses separately from other cells. The marker therefore supports experimental workflows focused on hematopoiesis, immune development, and infection-related effects.
Both approaches use antibodies directed toward the CD34 surface marker, but they represent different enrichment formats. Magnetic separation is used to isolate or enrich cells through antibody-associated magnetic handling, whereas flow cytometry supports identification and selection based on measured cellular characteristics. Researchers can therefore choose between these approaches when preparing cells for functional or infection studies.
A typical preparation begins with identifying the population through CD34 surface expression, followed by enrichment using antibody-based magnetic separation or flow cytometry. The resulting cell population can then be examined for function, infection responses, or differentiation-related behavior. This workflow creates a more focused system for connecting cellular properties with blood formation and immune development.
Researchers can use these cells to examine whether infection or pathogen exposure alters their function, infection responses, or ability to generate blood-cell progenitors. Because the cells contribute to lymphoid and myeloid lineages, experiments can connect pathogen effects with later immune-cell production. Such studies help clarify how infection may influence hematopoiesis and host defense.
Their capacity for self-renewal and production of blood-cell progenitors makes CD34+ cells relevant to studies of restoring blood formation and immunity. Researchers can investigate how these cells contribute to immune reconstitution after disease or treatment, including the return of leukocyte-producing potential. This context links stem-cell biology with strategies for recovering immune-system function.