Cd8 Antibody Detection
Cd8 Antibody Detection - Related Videos
05:41
Nanoparticle-Based Enrichment and Expansion of Antigen-Specific CD8+ T Cells
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2025
This video describes a protocol for isolating rare-antigen-specific CD8+ T cells using magnetic nanoparticle-based artificial antigen-presenting cells (aAPCs) functionalized with antigenic peptide-loaded MHC-Igs and anti-CD28 antibodies. Interaction of the aAPCs with CD8+ T cells specifically expressing the antigenic peptide-targeting T cell receptors, followed by their expansion in vitro, results in the enrichment and expansion of this CD8+ T cell population.
06:20
An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells
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2025
This video demonstrates an assay to analyze antigen-specific CD8+ T cells in different cell cycle stages. Fluorescently-labeled viable cells are selected using flow cytometry, and the cells in the different cell cycle stages are identified by their DNA content using antibodies against cell proliferation-associated nuclear protein and a nucleic acid stain.
08:28
Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
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Cited by 21 •
2017
This protocol describes a method to image resident tumor-infiltrating CD8 T cells labeled with fluorescently coupled antibodies within human lung tumor slices. This technique permits real-time analyses of CD8 T cell migration using confocal microscopy.
04:25
A Peptide-MHC-I Tetramer Staining Technique to Analyze SIV-Specific CD8+ Memory T Cells
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2025
This video demonstrates a method for characterizing CD8+ memory T cells specific to simian immunodeficiency virus (SIV) using peptide-major histocompatibility complex class I (pMHC-I) tetramers. A pMHC-I tetramer is a complex of four MHC-I molecules bound to SIV-specific peptides and conjugated to a fluorophore. These tetramers selectively bind to T cell receptors (TCRs) on SIV-specific CD8+ T cells, facilitating the characterization of these cells.
05:04
An In Vitro Assay for Studying the Cytotoxicity of Pre-Activated CD8+ T Cells against Cancer Cells
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2025
This video showcases an apoptosis assay in cancer cells induced by pre-activated effector T cells. By co-culturing these cells with cancer cells marked with fluorescent nuclei, a caspase-cleavable fluorogenic substrate is added. Apoptotic cancer cells will display a different color in their nuclei, leading to dual fluorescence. This dual fluorescence is indicative of cancer cells undergoing apoptosis.
FAQs
The primary antibody recognizes CD8 on or within a cell, and its binding is converted into a detectable signal by a label or secondary reagent. Signal-associated measurements identify cells carrying the target antigen, allowing investigators to estimate the CD8-positive population and examine how that population changes during immune activation or infection.
Flow cytometry measures labeled cells individually, making it useful for quantifying CD8-positive populations and characterizing T-cell subsets. Immunostaining instead reveals where antibody-associated signal appears in cells or samples. The choice therefore depends on whether the study emphasizes population measurements or visual assessment of antigen localization and cellular distribution.
CD8 measurements provide an indicator of immune-cell populations associated with cytotoxic T lymphocytes. Examining the proportion or distribution of CD8-positive cells can add context to studies of cellular immunity, including changes linked to infection or immune activation. This information supports comparisons between biological conditions without relying only on broader measures of immune response.
Interpretation depends on whether the antibody binds the intended CD8 antigen and whether the attached label or secondary reagent produces a measurable signal. The assay format also matters because CD8 may be assessed on or within cells. These considerations affect whether results are suitable for identifying positive cells, estimating population changes, or examining staining patterns.
A typical workflow applies an antibody specific to CD8 to the cells or sample, allows antigen-associated binding, and uses either a detectable label or a secondary reagent to generate signal. The sample is then examined by flow cytometry or immunostaining. The resulting measurements or staining patterns are used to assess CD8-positive cells.
The method can provide measurements of CD8-positive cell populations and help characterize T-cell subsets. Depending on the selected readout, researchers may obtain population-level measurements through flow cytometry or visual staining information through immunostaining. These outcomes support evaluation of immune-cell composition and monitoring of changes associated with experimental conditions.
In infection research, CD8 measurements help examine host-pathogen interactions and changes in immune-cell populations during disease progression. In vaccine or intervention studies, the same approach can monitor alterations in CD8-positive cells after treatment or immune stimulation. These applications connect antigen-specific detection with broader assessments of cellular immune responses.