Dual Chamber System
Dual Chamber System - Related Videos
11:11
Canine Intestinal Organoids in a Dual-Chamber Permeable Support System
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Cited by 18 •
2022
Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.
01:16
Chambers of the Heart
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2025
The human heart is a complex organ made up of four chambers: the right and left atria and the right and left ventricles. These internal chambers are separated by partitions known as the interatrial and interventricular septa. The exterior of the heart features a groove known as the coronary sulcus that demarcates the atria from the ventricles, while the anterior and posterior interventricular sulci distinguish between the two ventricles. Deoxygenated blood from the body is received in the right...
07:33
In vivo Dual Substrate Bioluminescent Imaging
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Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
07:33
A Customizable Chamber for Measuring Cell Migration
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Cited by 2 •
2017
This protocol details a customizable method to measure cell migration in response to chemoattractants that may also be used to determine the diffusion rate of a drug out of a polymer matrix.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
FAQs
The membrane’s permeability determines which signals can move between compartments. Soluble factors and nutrients may cross while the primary cultures remain physically separated, and some membrane designs also permit cell migration. This selective exchange allows investigators to examine communication across a barrier without losing the ability to attribute cellular responses to a particular compartment.
Physical separation helps distinguish direct cell contact from effects caused by soluble signaling. Researchers can therefore assess whether one compartment changes the behavior of another through released factors, exchanged nutrients, or migrating cells. This distinction is especially useful when analyzing inflammatory signaling or host-pathogen interactions in which several communication routes may operate simultaneously.
Barrier disruption can change the movement of soluble factors, nutrients, or cells between compartments, increasing communication beyond the intended baseline. That shift may indicate altered barrier function during infection or inflammation, but it can also complicate interpretation if the experiment aims to measure controlled signaling. Comparing compartment-specific observations helps relate changes to barrier integrity and cellular responses.
A typical setup places the relevant primary cultures or tissues in separate chambers divided by a porous membrane. The system is then maintained under defined conditions that permit the intended exchange between compartments. Researchers can sample each side independently during or after the experiment, preserving information about how each compartment responds to the shared biological environment.
This approach is useful when the research question concerns interactions across a biological barrier rather than isolated behavior within one culture. Applications described for the system include modeling host-pathogen encounters, epithelial or endothelial barriers, immune-cell recruitment, and inflammatory signaling. Its compartmental design supports controlled investigation of how infection-related signals influence neighboring cells or tissues.
Independent sampling from each chamber helps connect a stimulus or infection-related event with responses in the exposed and neighboring compartments. Investigators can examine communication across the membrane while retaining the identity of the source and receiving side. This organization supports analysis of infection mechanisms, barrier disruption, and immune responses without combining signals from both cultures into one sample.