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Immunology and Infection
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Canine Intestinal Organoids in a Dual-Chamber Permeable Support System
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

DOI:
10.3791/63612-v

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11:11 min

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March 02, 2022

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, , , , , , , , , ,
1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration

Chapters

  • 00:04Introduction
  • 01:05Permeable Support Seeding
  • 05:30TEER Value Measurement
  • 06:51Experimental Layout
  • 08:09Results: Staining, Light Microscopy, and TEM Images of Canine Intestinal Organoid Monolayer
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.

Tags

Keywords: Canine Intestinal OrganoidsDual-chamber Permeable Support SystemBiomedical Research ModelsOral PermeabilityTherapeutic Drug CandidatesIn Vitro Tool2D Cell Lines3D Self-assembled Epithelial StructuresAdult Stem CellsMonolayer MaintenanceExtracellular MatrixTight JunctionsCell DifferentiationNuclear ReceptorsCaco-2MDCKGastrointestinal AnatomyIntestinal MicrofloraDrug PermeabilityParallel Drug Development

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