Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

Vojtech Gabriel; Christopher Zdyrski; Dipak K. Sahoo; Kimberly Dao; Agnes Bourgois-Mochel; Todd Atherly; Marilyn N. Martinez; Donna A. Volpe; Jamie Kopper; Karin Allenspach; Jonathan P. Mochel

doi:10.3791/63612

Journal

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Immunology and Infection

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Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

3,358 Views

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11:11 min

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March 02, 2022

DOI:

10.3791/63612-v

DOI:

10.3791/63612-v

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11:11 min

March 02, 2022

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21 Views

Vojtech Gabriel¹, Christopher Zdyrski¹, Dipak K. Sahoo², Kimberly Dao³, Agnes Bourgois-Mochel², Todd Atherly³, Marilyn N. Martinez⁴, Donna A. Volpe⁵, Jamie Kopper², Karin Allenspach^2,3, Jonathan P. Mochel^1,3

¹Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, ²Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, ³3D Health Solutions Inc., ⁴Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, ⁵Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration

Transcript

The combination of canine organoid technology and a dual chamber permeable support system overcomes the limitations of experimental 2D cell lines traditionally used in biomedical research models.The technology can increase the reliability of predictions of the apparent oral permeability of therapeutic drug candidates both in canine and human patients.The system can be applied to other fields of biomedical research including microbiology research, viral uptake studies, drug-drug interactions, and drug transport mechanisms.The standardization, detailed documentation of experimental procedures and consistent monitoring of the cell monolayers are crucial practices needed for the permeable support assays.Place the extracellular membrane matrix on ice to begin thawing.Submerge it in ice to protect against rapid thawing and avoid solidification.Pre-chill a refrigerated centrifuge to 4

Summary

Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.