Fecal Rna Isolation
Fecal Rna Isolation - Related Videos
05:35
Fecal (micro) RNA Isolation
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2020
This protocol isolates high quality total RNA from fecal samples of animal and human subjects. A commercial miRNA isolation kit is used with significant adaption to isolate pure RNA with optimized quantity and quality. The RNA isolates are good for most downstream RNA assays such as sequencing, micro-array, and RT-PCR.
10:58
Isolation of Fecal Bacteria from Water Samples by Filtration
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events include parasites, bacteria, and viruses that are shed in high numbers in the feces of infected people and animals. Transmission to new and susceptible hosts may...
09:43
Isolation and Characterization of RNA-Containing Exosomes
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Cited by 515 •
2012
This paper demonstrates methods for the isolation, purification and detection of exosomes, as well as techniques for analysis of their molecular content. These methods are adaptable for exosome isolation from both cell culture media and biological fluids, and can beyond analysis of molecular content also be useful in functional studies.
02:49
Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates
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2025
This video demonstrates the detection of bacteriocin-producing lactic acid bacteria derived from mouse fecal matter. The assay determines the antimicrobial activity of bacteriocin on specific bacterial strains.
07:32
Murine Fecal Isolation and Microbiota Transplantation
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Cited by 4 •
2023
The goal here is to outline a protocol to investigate the mechanisms of dysbiosis in cardiovascular disease. This paper discusses how to aseptically collect and transplant murine fecal samples, isolate intestines, and use the "Swiss-roll" method, followed by immunostaining techniques to interrogate changes in the gastrointestinal tract.
FAQs
Mechanical disruption breaks apart stool-associated particles and cells, while chemical lysis helps release their contents into the surrounding solution. Combining these actions improves access to RNA from host material, microorganisms, and viruses. The coordinated treatment is important because incomplete disruption can reduce recovery, whereas effective lysis supports subsequent protection, clarification, and purification of the RNA.
Nuclease inhibition limits enzymatic RNA degradation after the sample is disrupted. Clarification then removes insoluble material and other components from the lysate before purification. Together, these steps help preserve RNA integrity and reduce the carryover of stool-derived substances that could interfere with downstream analysis, including reverse transcription and detection of target transcripts.
The recovered RNA can represent host immune transcripts, microbial gene expression, or pathogen-associated RNA, depending on the research question and analytical targets. This makes the method useful for examining either the host response or molecular signals associated with microorganisms and infection. Distinguishing these target types helps researchers interpret intestinal samples in a biologically relevant context.
A typical workflow begins with mechanical disruption and chemical lysis, followed by nuclease inhibition to protect released RNA. The lysate is clarified, and purification removes proteins and compounds that can inhibit amplification or reverse transcription. The isolated material can then enter downstream molecular analysis. Each stage addresses a different challenge created by the complexity of stool.
Researchers can use the method when they need molecular information from stool about infection status, pathogen-associated RNA, or changes in intestinal biology. The resulting RNA supports reverse transcription and downstream analysis rather than serving only as a recovered sample. This enables molecular detection and helps connect signals in the intestinal environment with infection-related processes.
In immunology and infection research, isolated RNA can be analyzed for host immune transcripts alongside microbial or pathogen-associated signals. Comparing these molecular readouts helps relate intestinal immune responses to the presence or status of infection. The approach also supports biomarker studies, where RNA patterns may provide measurable indicators of biological or disease-related conditions.