Nanofluidic Device
Nanofluidic Device - Related Videos
11:13
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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Cited by 1 •
2016
We propose a simple self-assembly technique of silica colloidal nanoparticles to create a nanofluidic junction between two microchannels in polydimethylsiloxane (PDMS). Using this technique, a nanoporous bead membrane with a pore size down to ~45 nm was built inside a microchannel and applied to electrokinetic preconcentration of DNA samples.
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
08:29
Thermal Measurement Techniques in Analytical Microfluidic Devices
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Cited by 6 •
2015
Here, we present three protocols for thermal measurements in microfluidic devices.
07:23
Fabrication of Nanoheight Channels Incorporating Surface Acoustic Wave Actuation via Lithium Niobate for Acoustic Nanofluidics
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Cited by 5 •
2020
We demonstrate fabrication of nanoheight channels with the integration of surface acoustic wave actuation devices upon lithium niobate for acoustic nanofluidics via liftoff photolithography, nano-depth reactive ion etching, and room-temperature plasma surface-activated multilayer bonding of single-crystal lithium niobate, a process similarly useful for bonding lithium niobate to oxides.
07:32
Synthesis of Graphene Nanofluids with Controllable Flake Size Distributions
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2019
A method for synthesizing graphene nanofluids with controllable flake size distributions is presented.
FAQs
Surface charge affects how ions and other charged or polarizable species interact with channel walls, while electrokinetic transport moves fluid and molecules under electrically driven conditions. Together with diffusion, these effects determine whether proteins, nucleic acids, or other analytes are retained, separated, or transported efficiently. Their influence becomes especially important when measurements depend on controlled molecular movement through confined spaces.
Molecular confinement restricts movement to dimensions comparable to the analytes themselves, making diffusion and surface interactions more influential than they are in larger channels. This can support detailed examination of individual molecules or very small quantities of material. In infection research, such control is relevant to analyzing pathogen-specific nucleic acids and distinguishing molecular signals within limited samples.
These systems require only small reagent and sample volumes because their channels or pores operate at molecular dimensions. Their controlled transport can allow several targets to be processed or analyzed within one platform, supporting multiplexed measurements. That combination is useful when samples are scarce, when multiple pathogen or immune markers must be examined, or when rapid testing is desirable.
A general workflow begins with introducing a small biological sample into the device, controlling how its molecular contents move through nanoscale channels or pores, and analyzing the resulting transport behavior or molecular measurements. The selected targets may include pathogens, immune biomarkers, or pathogen-specific nucleic acids. The outcome is a compact analysis that can support rapid or multiplexed detection.
Nanofluidic platforms can be directed toward several target classes identified in the source context: whole pathogens, immune biomarkers, and nucleic acids specific to pathogens. This range connects the technology to both infectious-agent detection and host-response analysis. Examining these targets with very small samples can help researchers investigate infection while preserving the possibility of rapid, multiplexed measurements.
Their value extends beyond detecting whether a pathogen is present. By controlling and analyzing proteins, nucleic acids, and other molecular contents in confined spaces, these devices can support detailed studies of interactions between infectious agents and host immune responses. Single-molecule measurements may provide especially fine-grained information, while compact formats can connect mechanistic research with point-of-care diagnostic development.