Human Enteroid Model
Human Enteroid Model - Related Videos
05:31
Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model
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2025
This video demonstrates the development of necrotizing enterocolitis disease in a human-derived neonatal enteroid model induced by lipopolysaccharides treatment. This model helps to study intestinal pathophysiology.
08:42
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
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Cited by 24 •
2019
Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.
06:33
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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Cited by 132 •
2015
We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.
06:51
Microfluidic Model of Necrotizing Enterocolitis Incorporating Human Neonatal Intestinal Enteroids and a Dysbiotic Microbiome
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Cited by 5 •
2023
This protocol describes an in vitro model of necrotizing enterocolitis (NEC), which can be used for mechanistic studies into disease pathogenesis. It features a microfluidic chip seeded with intestinal enteroids derived from the human neonatal intestine, endothelial cells, and the intestinal microbiome of a neonate with severe NEC.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
FAQs
Human intestinal stem cells proliferate within a supportive culture environment and self-organize into a three-dimensional epithelial tissue. This process produces a polarized structure containing multiple intestinal cell types rather than a uniform cell layer. The resulting organization is important because it preserves key features of intestinal structure and function needed for controlled biological studies.
Epithelial polarity creates an organized tissue with distinct structural orientation, allowing researchers to examine barrier function and pathogen invasion in a setting that more closely reflects intestinal organization. This feature helps connect changes in epithelial structure with host-microbe interactions, making the model useful for investigating how infectious processes affect the gut lining.
The system supports investigation of epithelial barrier function, host-microbe interactions, pathogen invasion, and immune signaling. Examining these processes together can help researchers relate microbial exposure to epithelial changes and signaling responses. In immunology and infection studies, that integrated view contributes to understanding disease mechanisms rather than focusing only on the pathogen or the tissue independently.
A Human Enteroid Model provides a controlled laboratory system based on human intestinal tissue, allowing researchers to examine epithelial and infection-related processes without relying solely on animal models. Its human cellular context can complement animal studies by offering a focused way to investigate barrier behavior, pathogen interactions, and immune signaling under defined experimental conditions.
Generation begins with human intestinal stem cells placed in a supportive culture environment. The cells proliferate and then self-organize into a three-dimensional, polarized epithelial tissue containing multiple intestinal cell types. Once established, this organized model can serve as a controlled platform for examining intestinal function, microbial interactions, pathogen invasion, and immune signaling.
The model is suited to questions about how pathogens invade intestinal epithelium, how host-microbe interactions influence tissue behavior, and how epithelial barrier function changes during disease-related processes. It also enables examination of immune signaling and evaluation of antimicrobial or anti-inflammatory interventions, helping connect experimental exposure with disease mechanisms and tissue responses.
Researchers can use these tissues to examine the effects of antimicrobial or anti-inflammatory interventions in a controlled human intestinal context. Comparing model responses under relevant experimental conditions may help clarify whether an intervention influences pathogen-related processes, epithelial barrier behavior, or immune signaling. The approach therefore supports both disease-mechanism studies and early evaluation of therapeutic strategies.
Because the model is generated from human intestinal stem cells, it can support studies of responses associated with particular human intestinal sources. Researchers can use this framework to investigate variation in epithelial behavior, host-microbe interactions, or immune signaling across experimental models. Such comparisons may provide context for personalized intestinal responses that broader model systems cannot capture as directly.