Multiplex Pcr
Multiplex Pcr - Related Videos
10:15
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
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Cited by 21 •
2011
An inexpensive, high throughput method for simultaneous detection of up to 43 molecular targets is described. Applications of mPCR/RLB include microbial typing and detection of multiple pathogens from clinical samples.
03:41
Agarose Gel Electrophoresis of DNA Amplicons Post PCR: A Method to Analyze Products of Multiplex PCR and Evaluate PCR Reaction Success
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2025
In this video, we demonstrate the separation of bacterial PCR-amplified DNA using agarose gel electrophoresis. Agarose gel functions as a molecular sieve, enabling the negatively-charged DNA to migrate based upon their size under an applied electric field.
07:27
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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Cited by 13 •
2011
Here we describe an optimized multiplex reverse transcriptase quantitative PCR (qRT-PCR) protocol in combination with a microfluidic platform as a cost and time effective high-throughput screening tool for microRNA (miRNA) expression levels, especially when working with limited amounts of sample.
09:03
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
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Cited by 18 •
2013
We demonstrate a simple multiplex PCR assay for quick-screening and typing of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V for methicillin-resistant Staphylococcus aureus, and provide some of the vital steps and procedural nuances that make it successful for adapting this assay to individual laboratories.
11:44
Monochrome Multiplex Quantitative PCR Telomere Length Measurement
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Cited by 7 •
2024
Here we present a protocol for the measurement of relative telomere length (TL) using the monochrome multiplex quantitative polymerase chain reaction (MMqPCR) assay. The MMqPCR assay is a repeatable, efficient, and cost-effective method for measuring TL from human DNA in population-based studies.
FAQs
Each primer pair is designed to selectively bind a particular DNA target while operating within the shared thermal-cycling conditions of the reaction. During amplification, the matched primers direct copying of their corresponding sequences, allowing several targets to be processed together. This coordinated design is central to examining multiple genetic markers from one sample.
The resulting amplicons can be differentiated by their size, by target-specific probes, or by fluorescence. Size-based discrimination separates products according to their lengths, whereas probes or fluorescent signals provide target-associated identification. These approaches allow researchers to determine which genetic markers were detected after the shared amplification reaction.
Testing several pathogen-associated targets in one reaction helps reveal whether more than one infectious agent is present in the same sample. This is important for coinfection studies because analyzing targets separately could provide a narrower view of the sample. Multiplex PCR therefore supports broader pathogen screening within a single molecular analysis.
A typical workflow begins by selecting the pathogen, immune-response, or antimicrobial-resistance markers of interest and preparing corresponding primer pairs. The targets are then amplified together under shared thermal-cycling conditions. After amplification, the products are distinguished by size, probe, or fluorescence, producing a combined readout of the selected genetic markers.
Researchers may choose this approach when they need to examine several pathogens or genetic markers efficiently from limited sample material. The reaction conserves sample and reagents while reducing processing time compared with handling each target in separate analyses. This makes it useful for broader diagnostic screening and more comprehensive investigation of infectious disease.
In addition to identifying pathogens and coinfections, the method can assess markers associated with immune responses or antimicrobial resistance. Measuring these targets together connects infectious-agent detection with biologically relevant response or resistance information. The combined analysis can support a broader investigation of how an infection is characterized at the molecular level.