Neonatal Mouse Gavage
Neonatal Mouse Gavage - Related Videos
07:18
Oral Gavage in Neonatal Mouse Pups and Functional Assessment of Gut Barrier Integrity Using Ussing Chambers
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2026
This article describes: (1) oral gavage delivery of test molecules to neonatal mice as early as DOL 6; and (2) ex vivo evaluation of colonic epithelial barrier function in biopsies from DOL 10 to weaning (DOL 20) pups by assessing paracellular and transcellular permeability with Ussing chambers.
10:36
Probiotic Studies in Neonatal Mice Using Gavage
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Cited by 9 •
2019
This study details the process of gavaging precise amounts of probiotics to neonatal mice. The experimental set-up was optimized to include but is not limited to probiotic dosing, methods of administration, and quantification of bacteria in the intestines.
03:09
Gavaging Adult Zebrafish
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Cited by 80 •
2013
The increasing use of zebrafish as an animal model requires the development of effective methods for the delivery of known quantities of compounds and solutions. The gavage procedure described below allows for the oral delivery of precise volumes of solution reliably, safely and efficiently to adult zebrafish.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
02:53
Neonatal Mouse Ovary Isolation: A Surgical Procedure to Harvest Pair of Ovaries from Neonatal Mouse Model
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2025
In this video, we describe a surgical procedure to isolate ovaries from a neonatal mouse.
FAQs
By delivering a measured substance directly to the stomach, neonatal mouse gavage reduces variation caused by voluntary feeding. Investigators can coordinate administration with a defined experimental schedule and compare animals more consistently. This control is especially useful when microbial preparations, pathogens, therapeutics, or antigens must be evaluated against intestinal colonization, mucosal immune responses, or disease outcomes.
The route places the administered material into the gastrointestinal system, where researchers can examine interactions with the developing intestinal environment. In neonatal studies, this supports analysis of early host-microbe relationships, including whether microbial preparations or pathogens are associated with intestinal colonization and mucosal immune responses. The approach therefore connects exposure conditions with subsequent intestinal and immune outcomes.
Reliable results depend on controlling the administered amount and timing while using equipment suited to the newborn animal. Careful handling also matters because the tube must be guided through the mouth and esophagus to reach the stomach. Consistency in these conditions helps limit procedural variation, making differences in colonization, immune responses, or disease outcomes easier to interpret.
A general workflow includes preparing a measured substance, selecting age-appropriate equipment, carefully guiding a feeding tube through the mouth and esophagus, and delivering the material into the stomach. Investigators then monitor the planned experimental outcomes, such as intestinal colonization, mucosal immune responses, or disease-related changes. The overview supports this sequence without specifying substance-specific or animal-specific settings.
Researchers may choose this technique when voluntary feeding could introduce unwanted differences in how much substance each newborn receives or when administration must occur at a controlled time. Direct delivery supports more consistent exposure across animals. That advantage is relevant to studies testing microbial preparations, pathogens, therapeutics, or experimental antigens during early immune development.
The method can support investigations of intestinal colonization, mucosal immune responses, and disease outcomes after controlled exposure. In immunology and infection research, those measurements help characterize host-microbe interactions during early life. Depending on the experimental substance, the same delivery approach can be used to study microbial preparations, pathogens, therapeutics, or experimental antigens within a neonatal model.