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Probiotic Studies in Neonatal Mice Using Gavage
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Immunology and Infection
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JoVE Journal Immunology and Infection
Probiotic Studies in Neonatal Mice Using Gavage

Probiotic Studies in Neonatal Mice Using Gavage

DOI:
10.3791/59074-v

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10:36 min

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January 27, 2019

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, , , , , , , , , , , ,
1Department of Experimental Medicine,University of British Columbia, 2Department of Pediatrics,University of British Columbia, 3Department of Epidemiology and Pediatrics,University of Nebraska Medical Centre, 4Animal Care Services,University of British Columbia

Chapters

  • 00:00Title
  • 00:04Intra-esophageal Gavage of Neonatal Mouse – Day of Life 2
  • 05:19Collection of Intestinal Samples for Colonization Analysis
  • 08:38Lactobacillus plantarum Colonization with Gavage Everyday or Every Other Day
  • 09:03Lactobacillus plantarum Gavaged Mice Co-housed with Untreated Mice
  • 09:28Conclusion

Summary

Automatic Translation

Automatic Translation

This study details the process of gavaging precise amounts of probiotics to neonatal mice. The experimental set-up was optimized to include but is not limited to probiotic dosing, methods of administration, and quantification of bacteria in the intestines.

Tags

Keywords: ProbioticNeonatal MiceGavageIntestinal MicrobiomeIntra-esophageal GavageSterile CollectionRepresentative ResultsHolding NestProbiotic Dye SolutionFeeding NeedlesCross ContaminationAir Bubbles

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