Organoid Derived Culture
Organoid Derived Culture - Related Videos
03:41
CRC Organoid Culture: A Method to Obtain 3D Organoids from Colorectal Cancer Cells
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2023
This video describes the detailed protocol for generating 3D organoids from murine-model derived colorectal cancer cells by propagating them on artificial extracellular matrix coated plates. These CRC organoids can be used as a model to study the primary growth of human tumor cells and metastasis.
07:33
In vitro Organoid Culture of Primary Mouse Colon Tumors
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Cited by 44 •
2013
A simple method to establish primary murine colon tumor organoid is described. This method utilizes the feature that colon tumor cells survive and grow into organoids in media containing limited growth factors, whereas normal colon epithelial do not.
08:29
Establishment and Culture of Patient-Derived Breast Organoids
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Cited by 7 •
2023
A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.
07:40
Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
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Cited by 46 •
2017
Modeling human brain development has been hindered due to the unprecedented complexity of neural epithelial tissue. Here, a method for the robust generation of brain organoids to delineate early events of human brain development and to model microcephaly in vitro is described.
01:32
Three-Dimensional Culture Systems: Spheroids and Organoids
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2026
Three-dimensional (3D) culture systems, including spheroids and organoids, support New Approach Methodology (NAM)-aligned research by providing more physiologically relevant models than conventional two-dimensional cell cultures. Because they more closely resemble the structure and cellular interactions of tissues and organs, these systems can provide physiologically relevant data before advancing to animal studies. Spheroids as Disease and Drug Screening Models Spheroids are generated when one...
FAQs
Its value comes from combining accessibility with retained biological identity. After organoid cells are dissociated or transferred, defined culture conditions allow them to retain organ-specific characteristics while exposing individual cell populations, host receptors, and tissue barriers for direct study. This arrangement supports controlled examination of how microbes engage host tissue without discarding the organ-related features that make the model informative.
These features provide the points of contact needed to connect a microbial event with a tissue response. Host receptors can be examined in relation to pathogen entry, while tissue barriers help frame interactions at the host surface or within the model. Access to separate cell populations also supports analysis of epithelial and immune-cell responses rather than treating the culture as an undifferentiated mass.
Conventional cell lines provide useful controlled systems, but Organoid Derived Culture can retain organ-specific characteristics and tissue barriers that cell lines may not represent. This added physiological relevance is important when a study asks how infection affects tissue organization, host receptors, or coordinated epithelial and immune-cell responses. The choice therefore depends on whether tissue context is central to the research question.
In immunology and infection research, the model links microbial behavior to host biology. Researchers can examine pathogen entry and replication alongside epithelial or immune-cell responses and interactions between host tissues and microbes. Because these processes are studied in a controlled culture environment, investigators can focus on mechanistic relationships and compare responses under defined experimental conditions.
The workflow starts with a three-dimensional organoid and converts it into a more accessible culture format. Researchers either dissociate the organoid into cells or transfer organoid material into defined culture conditions. They then study the resulting culture for organ-specific features, host receptors, tissue barriers, and responses relevant to the experimental question. The selected route determines how directly particular cell populations can be examined.
Defined culture conditions provide a controlled setting in which transferred or dissociated organoid cells can retain organ-specific characteristics. They also establish the environment for examining pathogen entry, replication, and host responses. In infection studies, this control helps investigators relate observed effects to the interaction being investigated rather than to an unspecified culture environment.
These cultures are useful when researchers need to connect a pathogen or treatment with responses from a particular tissue context. Applications described for the model include mechanistic studies, therapeutic testing, and personalized disease modeling. In immunology and infection, the framework can be directed toward pathogen entry, replication, epithelial or immune-cell responses, and interactions between host tissues and microbes.
Depending on the experimental design, the culture can help reveal how pathogens enter tissue, replicate, and interact with host components, as well as how epithelial or immune cells respond. It can also expose the relevance of host receptors and tissue barriers. These observations help investigators evaluate mechanisms, test therapeutic ideas, or model disease responses in a more tissue-relevant system.