Rna Transfection
Rna Transfection - Related Videos
05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
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2023
This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
10:15
Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
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Cited by 5 •
2017
Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.
08:55
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
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Cited by 13 •
2012
This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.
07:28
An Introduction to Transfection
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2023
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell enables the expression, or production, of proteins using the cells own machinery, whereas insertion of RNA into a cell is used to down-regulate the production of a specific protein by stopping translation. While the site of action for transfected RNA is the cytoplasm, DNA must be transported to the nucleus for effective transfection. There, the...
08:04
Neuronal Transfection Methods
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2023
Transfection - the process of transferring genetic material into cells - is a powerful tool for the rapid and efficient manipulation of gene expression in cells. Because this method can be used to silence the expression of specific proteins or to drive the expression of foreign or modified proteins, transfection is an extremely useful tool in the study of the cellular and molecular processes that govern neuron function. However, mature neurons have a number of properties that make them...
FAQs
The RNA species determines the downstream effect. Delivered messenger RNA serves as a template for translation, allowing cells to produce a selected protein such as an antigen, cytokine, or viral factor. Small interfering RNA instead promotes targeted degradation of a transcript, making it suitable for reducing expression of a chosen host or infection-related gene.
Delivery systems address the cell membrane barrier in different ways. Lipid-based carriers transport the RNA toward cellular entry, while electrical pulses provide an alternative physical approach for introducing it into cells. Comparing these options can help investigators examine how delivery strategy affects whether the intended expression or transcript reduction is achieved.
The transient behavior of introduced RNA supports experiments focused on short-term cellular responses. Investigators can observe protein production, transcript reduction, or immune signaling after a defined intervention and compare responses across different RNA cargoes or experimental conditions. This makes the approach useful when researchers need a temporary change in cell behavior for analysis.
A typical workflow begins by selecting synthetic RNA according to the desired outcome, choosing a lipid-based carrier or electrical-pulse delivery, and exposing living cells to the RNA. Investigators then assess protein production, targeted transcript reduction, or an immune response. The readout should match whether the cargo is messenger RNA or small interfering RNA.
The method can introduce RNA encoding an antigen, cytokine, or viral factor so researchers can examine cellular responses to a defined molecular stimulus. Alternatively, small interfering RNA can reduce a selected host transcript and help investigate its contribution to immune pathways. These designs support analysis of innate sensing and antiviral responses.
In infection research, transient antigen expression can support evaluation of vaccine designs, while expression of viral factors helps model infection-related effects in cells. Targeted host-gene knockdown can reveal pathways relevant to antiviral defense. The same framework also supports therapeutic-strategy research by linking a chosen RNA cargo to a measurable cellular outcome.