Shuttle Donor Vector
Shuttle Donor Vector - Related Videos
04:25
CRISPR-based Shuttle Cloning: A High-throughput Cloning Method
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2025
We describe a protocol for a high-throughput cloning method, CRISPR-based shuttle cloning (CRISPRshuttle cloning), which allows the transfer of DNA fragments of interest between vectors without the need for PCR amplification of the DNA fragments.
09:00
Cargo Loading onto Kinesin Powered Molecular Shuttles
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Cited by 4 •
2010
Molecular shuttles consisting of functionalized microtubules gliding on surface-adhered kinesin motor proteins can serve as a nanoscale transport system. Here, the assembly of a typical shuttle system is described.
07:18
An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
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Cited by 1 •
2022
Electroporation is a rapid, broadly adopted method for introducing exogenous DNA into the genus Rickettsia. This protocol provides a useful electroporation method for the transformation of obligate intracellular bacteria in the genus Rickettsia.
07:17
Single Port Donor Nephrectomy
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Cited by 6 •
2011
Single port laparoscopic surgery is changing the standard of care in surgical care like nothing since the laparoscopic technique was introduced 20 years ago. We present out technique of single port donor nephrectomy using the Gelpoint device. We have successfully performed this surgery in 100 patients.
08:35
Shuttle Box Assay as an Associative Learning Tool for Cognitive Assessment in Learning and Memory Studies using Adult Zebrafish
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Cited by 6 •
2021
Learning and memory are potent metrics in studying either developmental, disease-dependent, or environmentally induced cognitive impairments. Most cognitive assessments require specialized equipment and extensive time commitments. However, the shuttle box assay is an associative learning tool that utilizes a conventional gel box for rapid and reliable assessment of adult zebrafish cognition.
FAQs
Replication elements help determine whether the construct can be maintained in a compatible plasmid or host cell. Their role is especially important when DNA must be preserved before transfer or recovered after propagation. Selecting compatible replication functions therefore supports stable handling of the insert across the intended cloning or expression systems.
Selectable markers help researchers identify cells or plasmids that retain the construct after a transfer or maintenance step. This narrows analysis to material likely to contain the intended DNA sequence rather than every exposed sample. In infection and immunology studies, that distinction supports more consistent production of pathogen proteins, immune-modulating proteins, or vaccine antigens.
Engineered recombination or restriction sites provide defined locations for handling the inserted DNA sequence. They can support its controlled recovery and movement into a recipient vector, reducing ambiguity about where transfer occurs. Their placement and compatibility with the receiving system influence whether the intended sequence can be transferred and subsequently analyzed.
A typical workflow begins with maintaining the defined DNA sequence in a compatible donor construct, followed by recovering or releasing the insert through the engineered transfer features. The sequence is then moved into a compatible recipient vector and maintained or expressed under controlled laboratory conditions. Researchers use the resulting construct for downstream analysis.
The recipient should be compatible with the donor construct and appropriate for the intended expression or maintenance objective. In immunology and infection research, this choice depends on whether the goal is to produce a pathogen gene product, an immune-modulating protein, or a vaccine antigen. Compatibility supports reliable analysis of the resulting biological material.
These constructs enable researchers to move selected pathogen genes or immune-relevant sequences into systems where their products can be examined. The resulting expression studies can support analysis of antigen production, host-pathogen interactions, and candidate vaccine or therapeutic strategies. Their value lies in linking controlled DNA transfer with measurable infection or immune-response questions.