Tissue Explants
Tissue Explants - Related Videos
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
10:57
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
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2017
Protocols for studying the embryonic and perinatal murine aorta using in vivo clonal analysis and fate mapping, aortic explants, and isolated smooth muscle cells are detailed here. These diverse approaches facilitate the investigation of the morphogenesis of the embryonic and perinatal aorta in normal development and the pathogenesis in disease.
06:25
Explant Culture for Developmental Studies
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2023
Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo approach allows researchers to manipulate and observe developing tissues in ways that are not possible in vivo. Once established, explant culture is frequently used to understand the role of genes and signaling molecules in organogenesis. This video will first introduce the basic principles of explant culture and demonstrate a protocol to...
05:33
Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
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2023
Organotypic primary tissue explant cultures, which include precision-cut slices, represent the three-dimensional (3-D) tissue architecture as well as the multicellular interactions of native tissue. This video describes the culturing of murine lung tumor-derived tissue slices using a rotating incubation unit, a system that enables intermittent exposure of tissues to oxygen and nutrients. The protocol describes the methodology to generate and culture precision-cut lung tumor slices for drug...
09:30
Using Avian Skin Explants to Study Tissue Patterning and Organogenesis
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Cited by 6 •
2023
Here we describe protocols for three types of avian embryonic skin explant cultures that can be used to examine tissue interactions, 4D imaging timelapse movie (3D plus time), global or local perturbation of molecular function, and systems biology characterization.
FAQs
Maintaining native cellular organization allows resident immune, epithelial, stromal, and vascular-associated cells to interact within the same tissue environment. These relationships can shape how pathogens, antigens, or inflammatory signals are sensed and how local responses develop. Consequently, measurements such as cytokine release, immune-cell recruitment, and tissue damage reflect coordinated tissue behavior rather than responses from isolated cell populations.
Several local populations can contribute simultaneously, including resident immune cells, epithelial cells, stromal cells, and vascular-associated cells. Their combined activity helps connect pathogen or antigen exposure with inflammatory signaling, recruitment, and tissue injury. This cellular diversity is important when the research question concerns communication among tissue compartments rather than the response of one purified cell type.
These exposures provide distinct ways to challenge the tissue and examine coordinated biological responses. Researchers can assess outputs such as cytokine release, cell recruitment, and tissue damage after introducing a pathogen, presenting an antigen, or applying an inflammatory signal. Comparing these readouts helps distinguish direct infection-related effects from broader immune or inflammatory regulation within the tissue.
Tissue Explants retain more local organization and cellular interactions than isolated cultures while avoiding the full complexity of a whole-animal experiment. This intermediate position supports controlled investigation of host-pathogen interactions, infection spread, immune regulation, and tissue injury. Findings can therefore provide physiological context that single-cell systems may miss while reducing reliance on animal models.
A typical study begins by preparing a fragment of the relevant organ or tissue and maintaining it outside the body so its local organization and functions remain available for analysis. The explant is then exposed to a pathogen, antigen, or inflammatory signal. Researchers subsequently measure coordinated outcomes, including cytokine release, cell recruitment, or tissue damage.
This approach is useful when investigators need to examine infection spread, host-pathogen interactions, or immune regulation in an intact tissue setting. It can also support evaluation of therapeutic effects by revealing how an intervention influences coordinated tissue responses. Because explants preserve multiple local cell types, they are suited to questions that extend beyond the behavior of isolated immune cells.