Aseptic Surgical Technique
Aseptic Surgical Technique - Related Videos
10:48
Aseptic Technique in Environmental Science
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner Aseptic technique is a fundamental skill widely practiced in the field of environmental microbiology that requires a balance of mindfulness and practice in the laboratory. Proper use of this technique reduces the likelihood of bacterial or fungal contamination of reagents, culture media, and environmental samples. Aseptic technique is also vital to ensure data integrity and...
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
07:06
Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model
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2025
This study presents a novel surgical technique for implanting a biomimetic artificial intervertebral disc (BioAID). The method ensures precise placement and stability, aiming to restore cervical spine function using a goat model. This technique shows promise for clinical application in both human and canine patients suffering from cervical spine myelopathy.
06:08
A Standardized Surgical Technique for Tessier Medial Transnasal Canthopexy
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2026
Medial canthopexy allows correction of the position and fixation of the medial canthal tendon. Due to the technical difficulties of this procedure, there is a need for a detailed protocol for educational purposes. Here, we describe a standardized reproducible technique for bilateral medial transnasal canthopexy by an orbitonasal approach.
FAQs
Its effectiveness depends on coordinated controls rather than one isolated action. Hand hygiene addresses microorganisms on the hands, sterile instruments and supplies limit contaminated materials, protective barriers separate people or clothing from the field, and controlled handling reduces opportunities for transfer. Maintaining these measures throughout surgery helps preserve the field as conditions change.
Sterility must be preserved throughout the procedure because contamination can occur after preparation has been completed. Continued attention to handling, instruments, supplies, barriers, and the surrounding field keeps the original infection-prevention measures effective. This ongoing control supports safer wound healing and helps reduce postoperative complications associated with invasive care.
Hand hygiene and protective barriers address different routes by which microorganisms might reach the surgical field. Hand hygiene reduces contamination associated with the hands, while barriers limit transfer from people or their clothing and other sources. Used together with sterile supplies and controlled handling, they create overlapping protections rather than relying on a single safeguard.
Sterile instruments are one component of infection prevention, but they cannot maintain a protected field by themselves. Microorganisms may still be introduced through hands, supplies, barriers, or uncontrolled handling. Aseptic surgical technique combines these controls and sustains them before and during the procedure, making the overall approach broader than instrument sterility alone.
Preparation includes attention to hand hygiene, sterile instruments and supplies, protective barriers, controlled handling, and the condition of the sterile field. These elements should be considered together before the procedure begins, because each supports contamination control in a different way. Organized preparation establishes the conditions needed to continue aseptic practice during surgery.
It is relevant wherever invasive procedures take place, including operating rooms, procedure suites, and other clinical settings. Consistent practice helps protect patients by minimizing contamination during care, supporting safer wound healing, and reducing postoperative complications. It also provides healthcare professionals with a structured infection-prevention approach that can be applied across different surgical environments.