Double-lumen Tubes
Double-lumen Tubes - Related Videos
06:10
A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood
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Cited by 1 •
2025
Platelet-rich plasma (PRP) is used in the treatment of equine musculoskeletal disorders. Different techniques yield PRP with varying platelet and leukocyte concentrations. This article describes a method for obtaining PRP from horse blood.
03:36
Gold Immunostaining of Exosomal Sections: A Technique to Visualize Diagnostic Markers in Exosomal Lumen
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2023
In this video, we demonstrate gold immunostaining of biomarkers localized within the lumen region of ultrathin exosomal sections. This technique enables early diagnosis of diseases by visualization of target molecules within the exosomes secreted by the diseased cells.
02:34
Quantification of Group B Streptococcus Load in the Mouse Vaginal Lumen via Swabbing and Chromogenic Culture
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2026
Source: Patras, K. A. & Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the quantification of vaginal Group B Streptococcus (GBS) load in a mouse model using swabbing and chromogenic culture. It outlines the steps involved in sample collection, plating, and colony counting to quantify GBS load in the mouse vaginal lumen.
03:21
Localized Delivery of Bacteria into the Intestinal Lumen of a Zebrafish Larva Using Microinjection
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2025
Source: Li, J. et al. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020)This video demonstrates the microinjection-based delivery of fluorescently labeled Clostridioides difficile into the intestinal lumen of an anesthetized zebrafish larva. It outlines the steps involved in larval preparation, bacterial injection, and fluorescence microscopy–based confirmation of bacterial localization within the intestine.
06:45
Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
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Cited by 4 •
2023
Here, we describe an improvement of the semi-in vitro (SIV) method for observing pollen tube guidance and reception in Arabidopsis thaliana, which increases the receptivity of ovules. The high-throughput SIV cum septum method may be coupled with gametophyte marker lines and genetically encoded biosensors to monitor the dynamic process of fertilization.
FAQs
Inflatable tracheal and bronchial cuffs create seals that keep gas directed through the intended channel. When the seal is effective, clinicians can ventilate one lung while isolating the other. This selective control supports one-lung ventilation and helps prevent blood or secretions from entering the nonoperative lung.
Separate lumens allow clinicians to manage the lungs as distinct ventilation territories rather than treating both as a single pathway. Oxygen and anesthetic gases can be directed to the selected lung, while the other lung is isolated when necessary. This arrangement is especially relevant when the operative side must remain accessible during thoracic care.
Malposition can disrupt the intended separation between lungs, causing inadequate ventilation or hypoxemia, meaning insufficient oxygen in the blood. It may also contribute to airway injury. Because these problems are not reliably excluded by device placement alone, clinicians typically combine clinical assessment with fiberoptic bronchoscopy to verify location.
After placement, clinicians typically assess the patient clinically and use fiberoptic bronchoscopy to confirm the tube’s position. The bronchoscope provides direct verification of how the lumens and bronchial opening are positioned, while clinical assessment helps identify functional problems. Confirmation matters because unrecognized malposition can produce inadequate ventilation, hypoxemia, or airway injury.
These tubes are particularly useful during thoracic procedures when surgical access requires one lung to be ventilated while the other is excluded from the operative field. Independent control of the lungs can improve exposure for the procedure and permits oxygen and anesthetic gases to be directed selectively. Their use is also relevant in anesthesia and critical care.
Isolating the nonoperative lung can help protect it from blood or secretions. The cuffs contribute by sealing the tracheal and bronchial pathways, limiting unwanted movement of material between the selected and isolated sides. This protective role is important when maintaining separation is as important as delivering ventilation.